Detailed information of TA system TA231851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1509757..1510334
• Replicon: chromosome
• Accession: NZ_CP090769
• Organism: Burkholderia multivorans strain 2007Ycf875II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z18_RS06445
• Protein ID: WP_249461355.1
• Coordinates: 1509969..1510334 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z18_RS06440
• Protein ID: WP_035956044.1
• Coordinates: 1509757..1509975 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z18_RS06420 (L0Z18_06420)	1505396..1506667	-	1272	WP_249461346.1	Zn-dependent hydrolase	-
L0Z18_RS06425 (L0Z18_06425)	1506660..1507199	-	540	WP_249461349.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z18_RS06430 (L0Z18_06430)	1507417..1508583	-	1167	WP_249461352.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z18_RS06435 (L0Z18_06435)	1508759..1509655	+	897	WP_105794177.1	LysR substrate-binding domain-containing protein	-
L0Z18_RS06440 (L0Z18_06440)	1509757..1509975	+	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z18_RS06445 (L0Z18_06445)	1509969..1510334	+	366	WP_249461355.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z18_RS06450 (L0Z18_06450)	1510430..1510618	+	189	WP_200053152.1	hypothetical protein	-
L0Z18_RS06455 (L0Z18_06455)	1510657..1510815	-	159	WP_200053153.1	hypothetical protein	-
L0Z18_RS06460 (L0Z18_06460)	1511065..1511628	+	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z18_RS06465 (L0Z18_06465)	1511747..1513345	+	1599	WP_249461358.1	alkyl hydroperoxide reductase subunit F	-
L0Z18_RS06470 (L0Z18_06470)	1513407..1514147	-	741	WP_249461361.1	hypothetical protein	-
L0Z18_RS06475 (L0Z18_06475)	1514271..1515170	-	900	WP_249461365.1	lipid A hydroxylase LpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13625.66 Da
• Isoelectric Point: 5.8683

>T231851 WP_249461355.1 NZ_CP090769:1509969-1510334 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDITVADRAVTIRKER
RIRLPDAIVWASAQVNGRLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231851 NZ_CP126970:c1733649-1733392 [Corynebacterium sp. LM112]
ATGCTCCTCGTGTGGGATGAAAACGCCTGGGCCGATTACCTCTGGTGGCAGGCCCAGGACAGAAAGACCCTGAAGCGCAT
CAATCTGCTCCTCAAGGACATCGTCCGGAACGGCAATGAGGGTATCGGGAAGCCCGAGCCTCTCAAGCATGACTTCGCCG
GATACTGGTTGCGCCGGATCACCGACGAGCATCGGCTCGTCTACAAACACACGGAGACAGAGGTCCGGATCGCCGCGTGC
CGGTATCACTATGGGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231851 WP_035956044.1 NZ_CP090769:1509757-1509975 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231851 NZ_CP126970:c1733911-1733654 [Corynebacterium sp. LM112]
ATGAGAACAATGAGTTACACGGAGTCGCGAGCCAACTACGCGAAAGTGCTCGATTCCGTCACCGATGATCGCGAGGCGGT
GGTGATCACCCGCGCCGGCCACGAGCCAGTGGTCATGGTGTCGCTCGCCGACTATGAATCACTCCGCGAGACCGCATACC
TCATGCGCTCCCCCGCCAATGCCCGACGCCTGTTCGACGCCATGGAACGGCTGGAGGGTGGACGCGGCGACGCCCACGAC
GTGATCGAGACCAACTAG

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45