Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | vapBC/VapC-VapB |
Location | 762976..763556 | Replicon | plasmid pRLG201 |
Accession | NC_011368 | ||
Organism | Rhizobium leguminosarum bv. trifolii WSM2304 |
Toxin (Protein)
Gene name | vapC | Uniprot ID | B6A148 |
Locus tag | RLEG2_RS35115 | Protein ID | WP_012556029.1 |
Coordinates | 762976..763359 (-) | Length | 128 a.a. |
Antitoxin (Protein)
Gene name | vapB | Uniprot ID | B6A149 |
Locus tag | RLEG2_RS25635 | Protein ID | WP_003590910.1 |
Coordinates | 763356..763556 (-) | Length | 67 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
RLEG2_RS25600 | 758190..759854 | + | 1665 | WP_012556025.1 | arylsulfatase | - |
RLEG2_RS25605 | 759926..760894 | + | 969 | WP_012556026.1 | formylglycine-generating enzyme family protein | - |
RLEG2_RS25615 | 761155..761341 | - | 187 | Protein_729 | helix-turn-helix transcriptional regulator | - |
RLEG2_RS34995 | 761407..761580 | - | 174 | WP_012556028.1 | hypothetical protein | - |
RLEG2_RS25625 | 762106..762684 | - | 579 | WP_080518751.1 | MerR family DNA-binding protein | - |
RLEG2_RS35115 | 762976..763359 | - | 384 | WP_012556029.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
RLEG2_RS25635 | 763356..763556 | - | 201 | WP_003590910.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
RLEG2_RS25640 | 763807..764538 | - | 732 | WP_012556030.1 | GntR family transcriptional regulator | - |
RLEG2_RS25645 | 764705..765835 | - | 1131 | WP_012556031.1 | sugar phosphate isomerase/epimerase | - |
RLEG2_RS25650 | 765906..766778 | - | 873 | WP_012556032.1 | amino acid ABC transporter permease | - |
RLEG2_RS25655 | 766784..767716 | - | 933 | WP_012556033.1 | ABC transporter substrate-binding protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..1266105 | 1266105 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 128 a.a. Molecular weight: 14176.25 Da Isoelectric Point: 6.6041
>T23185 WP_012556029.1 NC_011368:c763359-762976 [Rhizobium leguminosarum bv. trifolii WSM2304]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRGSVMAFLAAQREAFVAAHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHTPDNSRN
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRGSVMAFLAAQREAFVAAHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHTPDNSRN
Download Length: 384 bp
>T23185 NC_011368:c763359-762976 [Rhizobium leguminosarum bv. trifolii WSM2304]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTAATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCGGTAGCGTGATGGCTTTCC
TGGCGGCCCAGCGTGAAGCGTTCGTCGCGGCGCACGACGAAGTTATGATGATGATTGATCGCCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGACCAACGAGCGGCCCTGTGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTGCACACCCCTGACAACTCGCGAAACTAA
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTAATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCGGTAGCGTGATGGCTTTCC
TGGCGGCCCAGCGTGAAGCGTTCGTCGCGGCGCACGACGAAGTTATGATGATGATTGATCGCCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGACCAACGAGCGGCCCTGTGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTGCACACCCCTGACAACTCGCGAAACTAA
Antitoxin
Download Length: 67 a.a. Molecular weight: 7140.23 Da Isoelectric Point: 11.3760
>AT23185 WP_003590910.1 NC_011368:c763556-763356 [Rhizobium leguminosarum bv. trifolii WSM2304]
MRSTINLDDTLMERARSLTGTKETATLVRQALETLVRVESGKRLVALGGTMPDAEAAPRRRSAAAK
MRSTINLDDTLMERARSLTGTKETATLVRQALETLVRVESGKRLVALGGTMPDAEAAPRRRSAAAK
Download Length: 201 bp
>AT23185 NC_011368:c763556-763356 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGCGATCGACTATCAATCTCGACGACACCCTCATGGAAAGGGCTAGGTCTCTAACGGGCACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCGTCGCGCTCGGTGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTGCCAAGTGA
ATGCGATCGACTATCAATCTCGACGACACCCTCATGGAAAGGGCTAGGTCTCTAACGGGCACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCGTCGCGCTCGGTGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTGCCAAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | B6A148 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7W9ZML6 |