Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 762976..763556 Replicon plasmid pRLG201
Accession NC_011368
Organism Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)


Gene name vapC Uniprot ID B6A148
Locus tag RLEG2_RS35115 Protein ID WP_012556029.1
Coordinates 762976..763359 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B6A149
Locus tag RLEG2_RS25635 Protein ID WP_003590910.1
Coordinates 763356..763556 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLEG2_RS25600 758190..759854 + 1665 WP_012556025.1 arylsulfatase -
RLEG2_RS25605 759926..760894 + 969 WP_012556026.1 formylglycine-generating enzyme family protein -
RLEG2_RS25615 761155..761341 - 187 Protein_729 helix-turn-helix transcriptional regulator -
RLEG2_RS34995 761407..761580 - 174 WP_012556028.1 hypothetical protein -
RLEG2_RS25625 762106..762684 - 579 WP_080518751.1 MerR family DNA-binding protein -
RLEG2_RS35115 762976..763359 - 384 WP_012556029.1 type II toxin-antitoxin system VapC family toxin Toxin
RLEG2_RS25635 763356..763556 - 201 WP_003590910.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
RLEG2_RS25640 763807..764538 - 732 WP_012556030.1 GntR family transcriptional regulator -
RLEG2_RS25645 764705..765835 - 1131 WP_012556031.1 sugar phosphate isomerase/epimerase -
RLEG2_RS25650 765906..766778 - 873 WP_012556032.1 amino acid ABC transporter permease -
RLEG2_RS25655 766784..767716 - 933 WP_012556033.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..1266105 1266105


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14176.25 Da        Isoelectric Point: 6.6041

>T23185 WP_012556029.1 NC_011368:c763359-762976 [Rhizobium leguminosarum bv. trifolii WSM2304]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRGSVMAFLAAQREAFVAAHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHTPDNSRN

Download         Length: 384 bp

>T23185 NC_011368:c763359-762976 [Rhizobium leguminosarum bv. trifolii WSM2304]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTAATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCGGTAGCGTGATGGCTTTCC
TGGCGGCCCAGCGTGAAGCGTTCGTCGCGGCGCACGACGAAGTTATGATGATGATTGATCGCCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGACCAACGAGCGGCCCTGTGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTGCACACCCCTGACAACTCGCGAAACTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7140.23 Da        Isoelectric Point: 11.3760

>AT23185 WP_003590910.1 NC_011368:c763556-763356 [Rhizobium leguminosarum bv. trifolii WSM2304]
MRSTINLDDTLMERARSLTGTKETATLVRQALETLVRVESGKRLVALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT23185 NC_011368:c763556-763356 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGCGATCGACTATCAATCTCGACGACACCCTCATGGAAAGGGCTAGGTCTCTAACGGGCACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCGTCGCGCTCGGTGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B6A148

Antitoxin

Source ID Structure
AlphaFold DB A0A7W9ZML6

References