Detailed information of TA system TA231847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 50430..51076
• Replicon: plasmid p1_2008Ycf542
• Accession: NZ_CP090768
• Organism: Burkholderia multivorans strain 2008Ycf542

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Y85_RS30125
• Protein ID: WP_012211060.1
• Coordinates: 50430..50837 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Y85_RS30130
• Protein ID: WP_009692911.1
• Coordinates: 50834..51076 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y85_RS30090 (L0Y85_30090)	46714..47445	-	732	WP_009692920.1	hypothetical protein	-
L0Y85_RS30095 (L0Y85_30095)	47442..47750	-	309	WP_009692919.1	hypothetical protein	-
L0Y85_RS30100 (L0Y85_30100)	47761..47946	-	186	WP_009692918.1	hypothetical protein	-
L0Y85_RS30105 (L0Y85_30105)	48058..48978	-	921	WP_009692917.1	serine protease	-
L0Y85_RS30110 (L0Y85_30110)	48971..49129	-	159	WP_009692916.1	hypothetical protein	-
L0Y85_RS30115 (L0Y85_30115)	49126..49734	-	609	WP_012211062.1	hypothetical protein	-
L0Y85_RS30120 (L0Y85_30120)	49766..50086	-	321	WP_012211061.1	hypothetical protein	-
L0Y85_RS30125 (L0Y85_30125)	50430..50837	-	408	WP_012211060.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Y85_RS30130 (L0Y85_30130)	50834..51076	-	243	WP_009692911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Y85_RS30135 (L0Y85_30135)	51178..52146	-	969	WP_231856867.1	non-homologous end-joining DNA ligase	-
L0Y85_RS30140 (L0Y85_30140)	52703..53374	+	672	WP_012211058.1	AAA family ATPase	-
L0Y85_RS30145 (L0Y85_30145)	53374..54339	+	966	WP_012211057.1	ParB/RepB/Spo0J family partition protein	-
L0Y85_RS30150 (L0Y85_30150)	55084..55941	+	858	WP_012211056.1	plasmid replication initiator TrfA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..156151	156151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (12-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14613.77 Da
• Isoelectric Point: 5.1402

>T231847 WP_012211060.1 NZ_CP090768:c50837-50430 [Burkholderia multivorans]
MSLYLLDTNILSNVIRDPRGACASRIGETRPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHAMAVDAVLVTDNTAEFTRVTGLAVENWLRPA

Nucleotide

• Length: 408 bp

>T231847 NZ_CP126969:1433792-1434046 [Corynebacterium sp. R4]
ATGAAGACCATCTCGTACACCGAATCACGGAAACGGTACGCCGAAGTACTGGATCACGTCACCGACAACCGCGAACCCGT
TATCGTCACCCGCGCGGGTCACGAATCGGTTGTTGTGATGGCTCTCGACGATTTCAATTCCATGCAGGAAACGGCGTATC
TCATGCGCTCCCCTCGCAATGCACAGCGACTCTTTGAATCCCTCGAGGAAGTCCGCTCCGGAAAGACCACCCCGCACGAT
CTCATCGAGGATTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8615

>AT231847 WP_009692911.1 NZ_CP090768:c51076-50834 [Burkholderia multivorans]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT231847 NZ_CP126969:1434053-1434307 [Corynebacterium sp. R4]
GTGGTTCTGTCGTGGTCAACACATGGTTGGGTGGACTACCTGTACTGGCAGTCCACCGACAAGAAAGTGCTCAAGCGGAT
CAACACCCTAATCAAAGACATTCCACGCAACGGCCACGAAGGCATTGGTAAGCCGGAGCCACTCAAACACGAGTTCTCAG
GCTATTGGTCACGACGAATCACCGACGAACACCGCCTCGTCTACTCCTACGCTGAGGATTCCATTGTGATCCTCAGCTGC
AGGTACCACTACTAG