Detailed information of TA system TA231844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1356424..1357463
• Replicon: chromosome
• Accession: NZ_CP090765
• Organism: Burkholderia multivorans strain 2008Ycf542

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A1W0YRD2
• Locus tag: L0Y85_RS05795
• Protein ID: WP_060096181.1
• Coordinates: 1356424..1356999 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: L0Y85_RS05800
• Protein ID: WP_054316743.1
• Coordinates: 1356996..1357463 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y85_RS05765 (L0Y85_05765)	1351814..1352896	+	1083	WP_006415045.1	porin	-
L0Y85_RS05770 (L0Y85_05770)	1353032..1353682	-	651	WP_244130927.1	hypothetical protein	-
L0Y85_RS05775 (L0Y85_05775)	1353810..1353920	+	111	Protein_1147	porin	-
L0Y85_RS05780 (L0Y85_05780)	1353979..1354944	-	966	WP_105836659.1	zinc-binding alcohol dehydrogenase family protein	-
L0Y85_RS05785 (L0Y85_05785)	1355023..1355274	-	252	WP_039218251.1	hypothetical protein	-
L0Y85_RS05790 (L0Y85_05790)	1355370..1356179	+	810	WP_105836660.1	helix-turn-helix transcriptional regulator	-
L0Y85_RS05795 (L0Y85_05795)	1356424..1356999	-	576	WP_060096181.1	PIN domain-containing protein	Toxin
L0Y85_RS05800 (L0Y85_05800)	1356996..1357463	-	468	WP_054316743.1	helix-turn-helix domain-containing protein	Antitoxin
L0Y85_RS05805 (L0Y85_05805)	1357579..1358385	-	807	WP_105836661.1	4-hydroxy-2-oxoheptanedioate aldolase	-
L0Y85_RS05810 (L0Y85_05810)	1358393..1359196	-	804	WP_105842135.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
L0Y85_RS05815 (L0Y85_05815)	1359211..1359603	-	393	WP_006403681.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
L0Y85_RS05820 (L0Y85_05820)	1359639..1360487	-	849	WP_012217098.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
L0Y85_RS05825 (L0Y85_05825)	1360530..1361993	-	1464	WP_105836663.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21554.67 Da
• Isoelectric Point: 5.0551

>T231844 WP_060096181.1 NZ_CP090765:c1356999-1356424 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLAAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDNLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T231844 NZ_CP126965:320124-320384 [Micrococcus lylae]
ATGATCAGATCGTTCGGAAGCAAGGACACCGAGCGCATCTGGCACGGGGCCTACGTGAAGCGCATCGATCGAACGGTGCA
GCGCGCGACCCTGAGGAAGTTGGAGATGATCAACGCCGCCCATGTGGTGGACGATCTCCGGATTCCACCGGGGAACCGCC
TGGAACAGCTCGTCGGTGACCGACGTGGTCAGTACAGCATCCGTGTCAATACGCAGTGGCGGCTCTGCTTCGTCTGGACT
GCTCTGCTTCGTCTGGACTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17581.02 Da
• Isoelectric Point: 6.1443

>AT231844 WP_054316743.1 NZ_CP090765:c1357463-1356996 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEGQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELGKQAQALRMGYE

Nucleotide

• Length: 468 bp

>AT231844 NZ_CP126965:320399-320719 [Micrococcus lylae]
ATGTCGAACTCGTCGACTGCCACTGACCTGGACCTGATCGAGCCCGTCCATCCGGGCGAGATCCTCTTGGCGGACTTCAT
CCAGGGCTTCGACCTGTCTCAGAACCGCGTGGCGGTCGCCCTGGGCGTCCCGCCGCGCCGCATCAACGAGATCGTCCTCG
GCAAGCGCGGCATCACCGCCGACACCGCCATTCGGTTGGCGCGCTACTTCGGCACATCGGAACAGTTCTGGATGAACCTT
CAGGTCGACTACGAGCTGCGCATGCAGCATCGGGTGCTCGACGGGCAGCTCGAGGAGATCCAGCCCCTGCAGGTCGCTTG
A

Structures

Toxin

• AlphaFold DB: A0A1W0YRD2