Detailed information of TA system TA023184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 177480..178165
• Replicon: plasmid pRLG201
• Accession: NC_011368
• Organism: Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B6A2X1
• Locus tag: RLEG2_RS22825
• Protein ID: WP_012555599.1
• Coordinates: 177743..178165 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B6A2X0
• Locus tag: RLEG2_RS22820
• Protein ID: WP_012555598.1
• Coordinates: 177480..177746 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG2_RS22800	173142..173831	+	690	WP_012555596.1	ABC transporter permease	-
RLEG2_RS22805	173952..174860	+	909	WP_003591853.1	homocysteine S-methyltransferase family protein	-
RLEG2_RS22810	174879..175316	-	438	WP_003591854.1	MarR family transcriptional regulator	-
RLEG2_RS22815	175522..177333	+	1812	WP_012555597.1	chloride channel protein	-
RLEG2_RS22820	177480..177746	+	267	WP_012555598.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RLEG2_RS22825	177743..178165	+	423	WP_012555599.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLEG2_RS22830	178205..178975	-	771	WP_012555600.1	ABC transporter ATP-binding protein	-
RLEG2_RS22835	178985..179968	-	984	WP_012555601.1	ABC transporter substrate-binding protein	-
RLEG2_RS22840	180009..180860	-	852	WP_012555602.1	ABC transporter permease	-
RLEG2_RS22845	180872..182224	-	1353	WP_012555603.1	LLM class flavin-dependent oxidoreductase	-
RLEG2_RS33465	182487..182714	+	228	Protein_170	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1266105	1266105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-60)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15544.76 Da
• Isoelectric Point: 4.9956

>T23184 WP_012555599.1 NC_011368:177743-178165 [Rhizobium leguminosarum bv. trifolii WSM2304]
VKLLLDTNVLSEVTKPNPDARVLEWLDKLDEDRSFISIVSVAEIRRGVALMDKGRKRDALAEWLAHDLTQRFEHRIIPVD
EPVALAWGDLMAQAKRSGRGLSSMDGLIAATAVAHDLGLATRNTRDFEGFGIELIDPWTG

Nucleotide

• Length: 423 bp

>T23184 NC_011368:177743-178165 [Rhizobium leguminosarum bv. trifolii WSM2304]
GTGAAGCTTCTGCTCGACACCAATGTCCTGTCCGAGGTGACGAAGCCGAACCCTGATGCGCGCGTCTTGGAATGGCTGGA
CAAGCTCGACGAGGATCGCAGCTTCATCAGCATCGTGTCGGTCGCAGAGATCCGGCGCGGTGTTGCCCTGATGGACAAGG
GACGGAAACGCGATGCGCTGGCCGAATGGCTTGCTCACGACCTGACGCAACGCTTCGAGCATCGCATCATTCCGGTGGAC
GAGCCGGTTGCCCTGGCATGGGGAGACCTTATGGCCCAGGCGAAGCGGAGCGGCCGCGGCCTGTCGTCAATGGACGGCCT
GATTGCGGCAACCGCGGTCGCTCACGACCTCGGCCTCGCGACGCGTAACACGAGGGATTTCGAGGGCTTCGGGATCGAAC
TGATCGATCCCTGGACAGGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9904.13 Da
• Isoelectric Point: 6.3726

>AT23184 WP_012555598.1 NC_011368:177480-177746 [Rhizobium leguminosarum bv. trifolii WSM2304]
MRPSRQGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTARKGTLVEFLMESPLRGADLDLERQR
DEPRDPDL

Nucleotide

• Length: 267 bp

>AT23184 NC_011368:177480-177746 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGCGGCCATCACGGCAAGGCGATGAAAGCTGGACTGTAGCCAGTGCAAAGGCGAAGCTTTCCGAGGTGATCGAACGCGC
GCAATCCGCGCCACAGACCATCACACGAAACGGCAAACCCAGCGTCGTCGTCGTTTCCGCCGAGGAATGGCAGCGGAAAA
CGGCGCGCAAGGGGACGCTTGTCGAATTCCTGATGGAATCGCCGCTTCGCGGCGCCGATCTGGACCTTGAGCGTCAGCGC
GACGAACCGCGTGATCCTGACCTGTGA

Structures

Toxin

• AlphaFold DB: B6A2X1

Antitoxin

• AlphaFold DB: B6A2X0