Detailed information of TA system TA231831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2789922..2790454
• Replicon: chromosome
• Accession: NZ_CP090763
• Organism: Burkholderia cenocepacia strain 2008Ycf657

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: L0Z06_RS17565
• Protein ID: WP_006484901.1
• Coordinates: 2790131..2790454 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: L0Z06_RS17560
• Protein ID: WP_012492929.1
• Coordinates: 2789922..2790134 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z06_RS17525 (L0Z06_17525)	2785379..2786674	+	1296	WP_012492927.1	Tol-Pal system beta propeller repeat protein TolB	-
L0Z06_RS17530 (L0Z06_17530)	2786692..2787204	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
L0Z06_RS17535 (L0Z06_17535)	2787224..2787973	+	750	WP_006484965.1	tol-pal system protein YbgF	-
L0Z06_RS17545 (L0Z06_17545)	2788400..2788720	-	321	WP_012492928.1	helix-turn-helix transcriptional regulator	-
L0Z06_RS17550 (L0Z06_17550)	2788859..2789254	+	396	WP_205671096.1	hypothetical protein	-
L0Z06_RS17555 (L0Z06_17555)	2789322..2789774	+	453	WP_006484947.1	hypothetical protein	-
L0Z06_RS17560 (L0Z06_17560)	2789922..2790134	+	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
L0Z06_RS17565 (L0Z06_17565)	2790131..2790454	+	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L0Z06_RS17570 (L0Z06_17570)	2790981..2791298	+	318	WP_226245292.1	hypothetical protein	-
L0Z06_RS17575 (L0Z06_17575)	2791423..2792334	+	912	WP_240465854.1	relaxase/mobilization nuclease domain-containing protein	-
L0Z06_RS17580 (L0Z06_17580)	2792457..2792900	-	444	WP_011544671.1	inner membrane protein YiaA	-
L0Z06_RS17585 (L0Z06_17585)	2793004..2793951	-	948	WP_205671098.1	AraC family transcriptional regulator	-
L0Z06_RS17590 (L0Z06_17590)	2794081..2794410	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
L0Z06_RS17595 (L0Z06_17595)	2794610..2795443	+	834	WP_034200961.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T231831 WP_006484901.1 NZ_CP090763:2790131-2790454 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T231831 NZ_CP126955:c4320785-4320555 [Escherichia coli O78:H4]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT231831 WP_012492929.1 NZ_CP090763:2789922-2790134 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT231831 NZ_CP126955:c4320558-4320199 [Escherichia coli O78:H4]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7