Detailed information of TA system TA231825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3161394..3161926
• Replicon: chromosome
• Accession: NZ_CP090760
• Organism: Burkholderia cenocepacia strain 2009Ycf95III

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: L0Z39_RS19355
• Protein ID: WP_006484901.1
• Coordinates: 3161603..3161926 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: L0Z39_RS19350
• Protein ID: WP_012492929.1
• Coordinates: 3161394..3161606 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z39_RS19315 (L0Z39_19315)	3156851..3158146	+	1296	WP_012492927.1	Tol-Pal system beta propeller repeat protein TolB	-
L0Z39_RS19320 (L0Z39_19320)	3158164..3158676	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
L0Z39_RS19325 (L0Z39_19325)	3158696..3159445	+	750	WP_006484965.1	tol-pal system protein YbgF	-
L0Z39_RS19335 (L0Z39_19335)	3159872..3160192	-	321	WP_012492928.1	helix-turn-helix transcriptional regulator	-
L0Z39_RS19340 (L0Z39_19340)	3160331..3160726	+	396	WP_205671096.1	hypothetical protein	-
L0Z39_RS19345 (L0Z39_19345)	3160794..3161246	+	453	WP_006484947.1	hypothetical protein	-
L0Z39_RS19350 (L0Z39_19350)	3161394..3161606	+	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
L0Z39_RS19355 (L0Z39_19355)	3161603..3161926	+	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L0Z39_RS19360 (L0Z39_19360)	3162453..3162770	+	318	WP_226245292.1	hypothetical protein	-
L0Z39_RS19365 (L0Z39_19365)	3162895..3163806	+	912	WP_240465854.1	relaxase/mobilization nuclease domain-containing protein	-
L0Z39_RS19370 (L0Z39_19370)	3163929..3164372	-	444	WP_011544671.1	inner membrane protein YiaA	-
L0Z39_RS19375 (L0Z39_19375)	3164476..3165423	-	948	WP_205671098.1	AraC family transcriptional regulator	-
L0Z39_RS19380 (L0Z39_19380)	3165553..3165882	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
L0Z39_RS19385 (L0Z39_19385)	3166082..3166915	+	834	WP_034200961.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T231825 WP_006484901.1 NZ_CP090760:3161603-3161926 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T231825 NZ_CP126955:c3475557-3475222 [Escherichia coli O78:H4]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT231825 WP_012492929.1 NZ_CP090760:3161394-3161606 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT231825 NZ_CP126955:c3475805-3475557 [Escherichia coli O78:H4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7