Detailed information of TA system TA231822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1362109..1363148
• Replicon: chromosome
• Accession: NZ_CP090758
• Organism: Burkholderia multivorans strain 2010Ycf31III

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: L0Z13_RS24625
• Protein ID: WP_045593318.1
• Coordinates: 1362109..1362684 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A8B3VE28
• Locus tag: L0Z13_RS24630
• Protein ID: WP_035946227.1
• Coordinates: 1362681..1363148 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z13_RS24595 (L0Z13_24595)	1357536..1358036	-	501	WP_226823143.1	hypothetical protein	-
L0Z13_RS24600 (L0Z13_24600)	1358050..1359108	-	1059	WP_226823145.1	DUF6402 family protein	-
L0Z13_RS24605 (L0Z13_24605)	1359492..1359605	+	114	Protein_1132	porin	-
L0Z13_RS24610 (L0Z13_24610)	1359664..1360629	-	966	WP_006409441.1	zinc-binding alcohol dehydrogenase family protein	-
L0Z13_RS24615 (L0Z13_24615)	1360708..1360959	-	252	WP_006409444.1	hypothetical protein	-
L0Z13_RS24620 (L0Z13_24620)	1361055..1361864	+	810	WP_006409450.1	helix-turn-helix transcriptional regulator	-
L0Z13_RS24625 (L0Z13_24625)	1362109..1362684	-	576	WP_045593318.1	PIN domain-containing protein	Toxin
L0Z13_RS24630 (L0Z13_24630)	1362681..1363148	-	468	WP_035946227.1	helix-turn-helix domain-containing protein	Antitoxin
L0Z13_RS24635 (L0Z13_24635)	1363264..1364070	-	807	WP_035487732.1	4-hydroxy-2-oxoheptanedioate aldolase	-
L0Z13_RS24640 (L0Z13_24640)	1364078..1364881	-	804	WP_006402377.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
L0Z13_RS24645 (L0Z13_24645)	1364896..1365288	-	393	WP_006409445.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
L0Z13_RS24650 (L0Z13_24650)	1365324..1366172	-	849	WP_006409439.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
L0Z13_RS24655 (L0Z13_24655)	1366215..1367678	-	1464	WP_006409448.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21555.65 Da
• Isoelectric Point: 4.8924

>T231822 WP_045593318.1 NZ_CP090758:c1362684-1362109 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLAAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDDLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T231822 NZ_CP126955:c2643046-2642795 [Escherichia coli O78:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17655.06 Da
• Isoelectric Point: 5.8709

>AT231822 WP_035946227.1 NZ_CP090758:c1363148-1362681 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEGQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELSKQAQDLRMGYE

Nucleotide

• Length: 468 bp

>AT231822 NZ_CP126955:c2642798-2642544 [Escherichia coli O78:H4]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B3VE28