Detailed information of TA system TA023182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3313611..3314284
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A9H385
• Locus tag: GDIA_RS15180
• Protein ID: WP_012227838.1
• Coordinates: 3313611..3314033 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A9H383
• Locus tag: GDIA_RS15185
• Protein ID: WP_012227836.1
• Coordinates: 3314030..3314284 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS15155	3309256..3310605	-	1350	WP_012554710.1	peptidoglycan DD-metalloendopeptidase family protein	-
GDIA_RS15160	3310607..3312157	-	1551	WP_012227846.1	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	-
GDIA_RS15165	3312260..3312643	+	384	WP_012554711.1	peptide-methionine (R)-S-oxide reductase MsrB	-
GDIA_RS15170	3312640..3313290	+	651	WP_012227842.1	uracil phosphoribosyltransferase	-
GDIA_RS15175	3313343..3313555	+	213	WP_012227840.1	dodecin family protein	-
GDIA_RS15180	3313611..3314033	-	423	WP_012227838.1	type II toxin-antitoxin system VapC family toxin	Toxin
GDIA_RS15185	3314030..3314284	-	255	WP_012227836.1	plasmid stabilization protein	Antitoxin
GDIA_RS15190	3314376..3315572	-	1197	WP_012554712.1	threonine ammonia-lyase	-
GDIA_RS15195	3315583..3316056	-	474	WP_012227833.1	transcription elongation factor GreA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15216.50 Da
• Isoelectric Point: 4.8485

>T23182 WP_012227838.1 NC_011365:c3314033-3313611 [Gluconacetobacter diazotrophicus PA1 5]
MILLDTNVVSEPWKPVPEPRVLEWIDAQAIETLWLSAVTVAELRFGIAAMPAGRRRSILHQSLEQDVLPLFDGRVLPFDL
AASQAYADLMMRARSDGRAIGQADGYIAAIAASRGYAVASRDVSPFEAAGVKILNPWQGA

Nucleotide

• Length: 423 bp

>T23182 NC_011365:c3314033-3313611 [Gluconacetobacter diazotrophicus PA1 5]
ATGATCCTTCTTGATACCAATGTGGTCTCGGAGCCATGGAAGCCTGTTCCTGAACCGCGTGTCCTGGAATGGATCGACGC
CCAGGCGATCGAGACGCTGTGGCTGTCGGCCGTCACGGTGGCGGAATTGCGATTCGGAATTGCCGCCATGCCGGCAGGCC
GGCGGCGTTCGATCCTGCATCAGAGTCTGGAACAGGACGTTCTGCCCCTGTTCGACGGGCGTGTCCTGCCCTTCGATCTG
GCGGCGTCCCAGGCTTATGCAGATCTGATGATGCGGGCCAGGTCCGATGGACGGGCGATCGGCCAGGCGGACGGCTATAT
TGCCGCAATTGCCGCATCCCGCGGTTACGCGGTGGCCAGTCGGGATGTGTCGCCGTTCGAGGCGGCCGGTGTGAAGATCC
TGAATCCGTGGCAGGGCGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9236.54 Da
• Isoelectric Point: 6.7262

>AT23182 WP_012227836.1 NC_011365:c3314284-3314030 [Gluconacetobacter diazotrophicus PA1 5]
MSSVVIRNLPEETHRALKARAALHGHSTEAEIRAILEAVVRPPKRVRLGSLLASIGREADVTEQDVAALQQVRDQTPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT23182 NC_011365:c3314284-3314030 [Gluconacetobacter diazotrophicus PA1 5]
ATGTCCTCTGTCGTCATCCGCAATCTTCCCGAAGAGACGCATCGGGCGCTGAAAGCGCGGGCCGCCCTGCATGGACACAG
CACGGAAGCGGAAATCCGCGCCATTCTGGAAGCCGTCGTCCGGCCGCCGAAGCGCGTCAGGCTGGGGTCGCTTCTGGCGT
CCATAGGTCGTGAAGCCGACGTGACCGAGCAGGATGTCGCGGCCTTGCAGCAGGTTCGGGATCAGACCCCAGCCGAGCCG
ATGACATTCGAATGA

Structures

Toxin

• AlphaFold DB: A9H385

Antitoxin

• AlphaFold DB: A9H383