Detailed information of TA system TA231818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 630249..631519
• Replicon: chromosome
• Accession: NZ_CP090758
• Organism: Burkholderia multivorans strain 2010Ycf31III

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z13_RS21515
• Protein ID: WP_006410613.1
• Coordinates: 630249..630827 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BS12
• Locus tag: L0Z13_RS21520
• Protein ID: WP_006406126.1
• Coordinates: 630962..631519 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z13_RS21495 (L0Z13_21495)	625537..626328	+	792	WP_035947285.1	enoyl-CoA hydratase	-
L0Z13_RS21500 (L0Z13_21500)	626325..627464	+	1140	WP_006410588.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z13_RS21505 (L0Z13_21505)	627511..628431	-	921	WP_006406123.1	LysR family transcriptional regulator	-
L0Z13_RS21510 (L0Z13_21510)	628610..630148	+	1539	WP_006410596.1	MFS transporter	-
L0Z13_RS21515 (L0Z13_21515)	630249..630827	-	579	WP_006410613.1	HD domain-containing protein	Toxin
L0Z13_RS21520 (L0Z13_21520)	630962..631519	-	558	WP_006406126.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z13_RS21525 (L0Z13_21525)	632032..633435	+	1404	WP_006406128.1	hypothetical protein	-
L0Z13_RS21530 (L0Z13_21530)	633446..634066	-	621	WP_006410617.1	isochorismatase family cysteine hydrolase	-
L0Z13_RS21535 (L0Z13_21535)	634655..635173	-	519	WP_227747530.1	antibiotic biosynthesis monooxygenase	-
L0Z13_RS21540 (L0Z13_21540)	635198..636223	-	1026	WP_006406131.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21384.10 Da
• Isoelectric Point: 4.9434

>T231818 WP_006410613.1 NZ_CP090758:c630827-630249 [Burkholderia multivorans]
MNPDTIRARLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
APEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDAAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAQT

Nucleotide

• Length: 579 bp

>T231818 NZ_CP126955:c2100554-2099232 [Escherichia coli O78:H4]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20378.30 Da
• Isoelectric Point: 7.8341

>AT231818 WP_006406126.1 NZ_CP090758:c631519-630962 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAAHLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMETALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231818 NZ_CP126955:c2100820-2100554 [Escherichia coli O78:H4]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: B9BS12