Detailed information of TA system TA231815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 995618..996318
• Replicon: chromosome
• Accession: NZ_CP090757
• Organism: Burkholderia multivorans strain 2010Ycf31III

Toxin (Protein)

• Gene name: tad
• Uniprot ID: B9BQB7
• Locus tag: L0Z13_RS07195
• Protein ID: WP_006405634.1
• Coordinates: 995941..996318 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: L0Z13_RS07190
• Protein ID: WP_006405635.1
• Coordinates: 995618..995941 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z13_RS07170 (L0Z13_07170)	990967..992427	+	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-
L0Z13_RS07175 (L0Z13_07175)	992586..994484	+	1899	WP_006409758.1	molecular chaperone HtpG	-
L0Z13_RS07180 (L0Z13_07180)	994493..995107	+	615	WP_006398690.1	chorismate lyase	-
L0Z13_RS07185 (L0Z13_07185)	995104..995595	+	492	WP_006409760.1	DNA-deoxyinosine glycosylase	-
L0Z13_RS07190 (L0Z13_07190)	995618..995941	-	324	WP_006405635.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z13_RS07195 (L0Z13_07195)	995941..996318	-	378	WP_006405634.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z13_RS07200 (L0Z13_07200)	996847..997101	-	255	WP_035488690.1	hypothetical protein	-
L0Z13_RS07205 (L0Z13_07205)	997329..998246	+	918	WP_006405631.1	spermidine synthase	-
L0Z13_RS07210 (L0Z13_07210)	998443..998676	+	234	WP_006398697.1	DUF3311 domain-containing protein	-
L0Z13_RS07215 (L0Z13_07215)	998673..1000223	+	1551	WP_006398698.1	sodium:solute symporter family protein	-
L0Z13_RS07220 (L0Z13_07220)	1000327..1000773	-	447	WP_006405630.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (23-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13564.63 Da
• Isoelectric Point: 9.7722

>T231815 WP_006405634.1 NZ_CP090757:c996318-995941 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKHLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGLGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLRAAEAHANGEK

Nucleotide

• Length: 378 bp

>T231815 NZ_CP126955:c984666-984448 [Escherichia coli O78:H4]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT231815 WP_006405635.1 NZ_CP090757:c995941-995618 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT231815 NZ_CP126955:c985066-984692 [Escherichia coli O78:H4]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: B9BQB7

Antitoxin

• AlphaFold DB: B9BQB8