Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-dnstrm_HI1420
Location 2020656..2021276 Replicon chromosome
Accession NZ_CP090754
Organism Burkholderia multivorans strain 2010Ycf41

Toxin (Protein)


Gene name relE Uniprot ID A0A0H3KJN2
Locus tag L0Z40_RS24645 Protein ID WP_012217495.1
Coordinates 2020656..2020973 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID B9B269
Locus tag L0Z40_RS24650 Protein ID WP_006398012.1
Coordinates 2020977..2021276 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Z40_RS24635 (L0Z40_24635) 2017189..2018622 - 1434 WP_006404214.1 aldehyde dehydrogenase family protein -
L0Z40_RS24640 (L0Z40_24640) 2018651..2020291 - 1641 WP_006398015.1 acetolactate synthase large subunit -
L0Z40_RS24645 (L0Z40_24645) 2020656..2020973 + 318 WP_012217495.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L0Z40_RS24650 (L0Z40_24650) 2020977..2021276 + 300 WP_006398012.1 putative addiction module antidote protein Antitoxin
L0Z40_RS24655 (L0Z40_24655) 2021311..2022597 - 1287 WP_053297867.1 DUF445 domain-containing protein -
L0Z40_RS24660 (L0Z40_24660) 2022712..2023932 - 1221 WP_105752934.1 anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase -
L0Z40_RS24665 (L0Z40_24665) 2023922..2024248 - 327 WP_006404219.1 anthranilate 1,2-dioxygenase ferredoxin subunit AndAb -
L0Z40_RS24670 (L0Z40_24670) 2024266..2024754 - 489 WP_006398008.1 anthranilate 1,2-dioxygenase small subunit AndAd -
L0Z40_RS24675 (L0Z40_24675) 2024769..2026040 - 1272 WP_105752935.1 anthranilate 1,2-dioxygenase large subunit AndAc -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(9-102)

Antitoxin

Uncharacterized protein HI1420

(4-96)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.94 Da        Isoelectric Point: 10.2349

>T231814 WP_012217495.1 NZ_CP090754:2020656-2020973 [Burkholderia multivorans]
MPYLAPTFSIRTTAVFDAWFAGLQDRTAKRRIQARIDRLSMGNLGNWKSVGSPVYEMRIDHGPGYRVYFVRRRTIWVVLL
CGGDKSTQRDDIRAAHAMLEHLDLE

Download         Length: 318 bp

>T231814 NZ_CP126955:274328-274678 [Escherichia coli O78:H4]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10605.00 Da        Isoelectric Point: 6.6381

>AT231814 WP_006398012.1 NZ_CP090754:2020977-2021276 [Burkholderia multivorans]
MSKISTRPWDSAEHLKTDDDMAEYFEACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Download         Length: 300 bp

>AT231814 NZ_CP126955:274083-274334 [Escherichia coli O78:H4]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3KJN2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3KTI5

References