Detailed information of TA system TA231808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3294806..3295443
• Replicon: chromosome
• Accession: NZ_CP090753
• Organism: Burkholderia multivorans strain 2010Ycf41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Z40_RS15485
• Protein ID: WP_035489546.1
• Coordinates: 3294806..3295207 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z40_RS15490
• Protein ID: WP_006408845.1
• Coordinates: 3295207..3295443 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z40_RS15475 (L0Z40_15475)	3292127..3293269	+	1143	WP_053296686.1	alginate lyase family protein	-
L0Z40_RS15480 (L0Z40_15480)	3293296..3294684	+	1389	WP_105751885.1	L-serine ammonia-lyase	-
L0Z40_RS15485 (L0Z40_15485)	3294806..3295207	-	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z40_RS15490 (L0Z40_15490)	3295207..3295443	-	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z40_RS15495 (L0Z40_15495)	3295631..3297334	+	1704	WP_105751886.1	thiamine pyrophosphate-binding protein	-
L0Z40_RS15500 (L0Z40_15500)	3297331..3298419	+	1089	WP_105751887.1	ADP-heptose--LPS heptosyltransferase	-
L0Z40_RS15505 (L0Z40_15505)	3298538..3299569	-	1032	WP_105751888.1	helix-turn-helix domain-containing protein	-
L0Z40_RS15510 (L0Z40_15510)	3299740..3300282	-	543	WP_088923753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231808 WP_035489546.1 NZ_CP090753:c3295207-3294806 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231808 NZ_CP126953:c89508-89230 [Escherichia coli O78:H4]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231808 WP_006408845.1 NZ_CP090753:c3295443-3295207 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231808 NZ_CP126953:c89777-89508 [Escherichia coli O78:H4]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1