Detailed information of TA system TA231807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 2065651..2066351
• Replicon: chromosome
• Accession: NZ_CP090753
• Organism: Burkholderia multivorans strain 2010Ycf41

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8E2RY82
• Locus tag: L0Z40_RS09600
• Protein ID: WP_070553238.1
• Coordinates: 2065651..2066028 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: L0Z40_RS09605
• Protein ID: WP_006405635.1
• Coordinates: 2066028..2066351 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z40_RS09575 (L0Z40_09575)	2061182..2061631	+	450	WP_105751509.1	VOC family protein	-
L0Z40_RS09580 (L0Z40_09580)	2061745..2063295	-	1551	WP_006398698.1	sodium:solute symporter family protein	-
L0Z40_RS09585 (L0Z40_09585)	2063292..2063525	-	234	WP_006398697.1	DUF3311 domain-containing protein	-
L0Z40_RS09590 (L0Z40_09590)	2063722..2064639	-	918	WP_053296988.1	spermidine synthase	-
L0Z40_RS09595 (L0Z40_09595)	2064867..2065121	+	255	WP_059940739.1	hypothetical protein	-
L0Z40_RS09600 (L0Z40_09600)	2065651..2066028	+	378	WP_070553238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z40_RS09605 (L0Z40_09605)	2066028..2066351	+	324	WP_006405635.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z40_RS09610 (L0Z40_09610)	2066374..2066865	-	492	WP_006398691.1	DNA-deoxyinosine glycosylase	-
L0Z40_RS09615 (L0Z40_09615)	2066862..2067476	-	615	WP_006398690.1	chorismate lyase	-
L0Z40_RS09620 (L0Z40_09620)	2067485..2069383	-	1899	WP_059875776.1	molecular chaperone HtpG	-
L0Z40_RS09625 (L0Z40_09625)	2069542..2071002	-	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13507.49 Da
• Isoelectric Point: 9.3081

>T231807 WP_070553238.1 NZ_CP090753:2065651-2066028 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGEK

Nucleotide

• Length: 378 bp

>T231807 NZ_CP126953:30795-31082 [Escherichia coli O78:H4]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT231807 WP_006405635.1 NZ_CP090753:2066028-2066351 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT231807 NZ_CP126953:30547-30798 [Escherichia coli O78:H4]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: B9BQB8