Detailed information of TA system TA231805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 9444..9921
• Replicon: plasmid p3_2010Ycf503
• Accession: NZ_CP090752
• Organism: Burkholderia multivorans strain 2010Ycf503

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: L0Z02_RS29850
• Protein ID: WP_249469239.1
• Coordinates: 9652..9921 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: L0Z02_RS29845
• Protein ID: WP_249469238.1
• Coordinates: 9444..9668 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z02_RS29820 (L0Z02_29820)	6310..6612	-	303	WP_249469262.1	hypothetical protein	-
L0Z02_RS29825 (L0Z02_29825)	6609..6899	-	291	WP_249469264.1	hypothetical protein	-
L0Z02_RS29830 (L0Z02_29830)	7208..7516	+	309	WP_005933969.1	hypothetical protein	-
L0Z02_RS29835 (L0Z02_29835)	7513..9189	+	1677	WP_249469236.1	relaxase/mobilization nuclease domain-containing protein	-
L0Z02_RS29840 (L0Z02_29840)	9146..9391	+	246	WP_249469266.1	hypothetical protein	-
L0Z02_RS29845 (L0Z02_29845)	9444..9668	+	225	WP_249469238.1	TraY domain-containing protein	Antitoxin
L0Z02_RS29850 (L0Z02_29850)	9652..9921	+	270	WP_249469239.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z02_RS29855 (L0Z02_29855)	9918..10196	+	279	WP_249469241.1	hypothetical protein	-
L0Z02_RS29860 (L0Z02_29860)	10276..10530	+	255	WP_249469242.1	hypothetical protein	-
L0Z02_RS29865 (L0Z02_29865)	10520..10840	+	321	WP_249469244.1	hypothetical protein	-
L0Z02_RS29870 (L0Z02_29870)	10876..11382	-	507	WP_249469246.1	hypothetical protein	-
L0Z02_RS29875 (L0Z02_29875)	11394..13586	-	2193	WP_249469248.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14081	14081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10481.22 Da
• Isoelectric Point: 9.4993

>T231805 WP_249469239.1 NZ_CP090752:9652-9921 [Burkholderia multivorans]
MAYRIEIDPAAERELDKLDREIAKRILRFLFERVAKLDDPRSIGEALKGSKLGEFWKYRVGDYRIIADIQDGVLCILVLR
VGNRREVYR

Nucleotide

• Length: 270 bp

>T231805 NZ_CP126953:c16567-16169 [Escherichia coli O78:H4]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8334.64 Da
• Isoelectric Point: 4.5881

>AT231805 WP_249469238.1 NZ_CP090752:9444-9668 [Burkholderia multivorans]
MLAIRLPPEVESRLDALAAATGRTKTFLAREAILDNLDRLEDVYLAEQRLVDIRAGRTEPIPLEDVMKKYGLPD

Nucleotide

• Length: 225 bp

>AT231805 NZ_CP126953:c16794-16567 [Escherichia coli O78:H4]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA