Detailed information of TA system TA231804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 68222..68817
• Replicon: plasmid p2_2010Ycf503
• Accession: NZ_CP090751
• Organism: Burkholderia multivorans strain 2010Ycf503

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: L0Z02_RS29680
• Protein ID: WP_059939298.1
• Coordinates: 68467..68817 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: L0Z02_RS29675
• Protein ID: WP_081068901.1
• Coordinates: 68222..68473 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z02_RS29650 (L0Z02_29650)	64560..65276	-	717	WP_217085077.1	translesion DNA synthesis-associated protein ImuA	-
L0Z02_RS29655 (L0Z02_29655)	65352..66266	-	915	WP_059939290.1	SOS response-associated peptidase family protein	-
L0Z02_RS29660 (L0Z02_29660)	66401..66724	-	324	WP_217085076.1	H-NS histone family protein	-
L0Z02_RS29665 (L0Z02_29665)	66999..67148	+	150	WP_155633434.1	hypothetical protein	-
L0Z02_RS29670 (L0Z02_29670)	67352..68032	+	681	WP_217085075.1	site-specific integrase	-
L0Z02_RS29675 (L0Z02_29675)	68222..68473	+	252	WP_081068901.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Z02_RS29680 (L0Z02_29680)	68467..68817	+	351	WP_059939298.1	type II toxin-antitoxin system ChpB family toxin	Toxin
L0Z02_RS29685 (L0Z02_29685)	68847..69122	+	276	WP_060097623.1	hypothetical protein	-
L0Z02_RS29690 (L0Z02_29690)	69139..69327	+	189	WP_217085074.1	hypothetical protein	-
L0Z02_RS29695 (L0Z02_29695)	69354..69812	+	459	WP_217085073.1	hypothetical protein	-
L0Z02_RS29700 (L0Z02_29700)	69836..70090	+	255	WP_217085072.1	hypothetical protein	-
L0Z02_RS29705 (L0Z02_29705)	70107..70376	+	270	WP_175795611.1	hypothetical protein	-
L0Z02_RS29710 (L0Z02_29710)	70606..71472	+	867	WP_249469179.1	hypothetical protein	-
L0Z02_RS29715 (L0Z02_29715)	71870..72990	+	1121	WP_105762551.1	IS3-like element IS407 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89642	89642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12444.41 Da
• Isoelectric Point: 8.9716

>T231804 WP_059939298.1 NZ_CP090751:68467-68817 [Burkholderia multivorans]
MVRRVKFERGDIVRVSLNPTIGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEARGARKIERAPAEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T231804 NZ_CP126952:c4950530-4950219 [Escherichia coli O78:H4]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8991.47 Da
• Isoelectric Point: 6.9769

>AT231804 WP_081068901.1 NZ_CP090751:68222-68473 [Burkholderia multivorans]
MELKIQKWGNSAAVRLPAVLLEQMHASVGGSLNADVRPDGVLLTPARRKYALEDLVAQCDVKAPVPADMAAWGEVKPVGR
EVW

Nucleotide

• Length: 252 bp

>AT231804 NZ_CP126952:c4950218-4949928 [Escherichia coli O78:H4]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG