Detailed information of TA system TA231801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1684309..1685579
• Replicon: chromosome
• Accession: NZ_CP090748
• Organism: Burkholderia multivorans strain 2010Ycf503

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z02_RS23770
• Protein ID: WP_249467910.1
• Coordinates: 1685001..1685579 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z02_RS23765
• Protein ID: WP_249467908.1
• Coordinates: 1684309..1684866 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z02_RS23750 (L0Z02_23750)	1680266..1681525	+	1260	WP_249467902.1	IS256-like element IS1356 family transposase	-
L0Z02_RS23755 (L0Z02_23755)	1681762..1682382	+	621	WP_249467904.1	isochorismatase family cysteine hydrolase	-
L0Z02_RS23760 (L0Z02_23760)	1682393..1683796	-	1404	WP_249467906.1	DUF308 domain-containing protein	-
L0Z02_RS23765 (L0Z02_23765)	1684309..1684866	+	558	WP_249467908.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z02_RS23770 (L0Z02_23770)	1685001..1685579	+	579	WP_249467910.1	HD domain-containing protein	Toxin
L0Z02_RS23775 (L0Z02_23775)	1685680..1687218	-	1539	WP_053298291.1	MFS transporter	-
L0Z02_RS23780 (L0Z02_23780)	1687397..1688317	+	921	WP_006406123.1	LysR family transcriptional regulator	-
L0Z02_RS23785 (L0Z02_23785)	1688364..1689503	-	1140	WP_006410588.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z02_RS23790 (L0Z02_23790)	1689500..1690291	-	792	WP_065496148.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1680266..1681525	1259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (6-161)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21504.22 Da
• Isoelectric Point: 5.5575

>T231801 WP_249467910.1 NZ_CP090748:1685001-1685579 [Burkholderia multivorans]
MNPDTIRARLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALHGIDTLKLLKLCVVHDLGEALHGDIP
APEQAAHPDKNMHERDDLLTLTAARDPVLRNELVALWDEYDVAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAHT

Nucleotide

• Length: 579 bp

>T231801 NZ_CP126952:4046187-4046564 [Escherichia coli O78:H4]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20325.28 Da
• Isoelectric Point: 8.5490

>AT231801 WP_249467908.1 NZ_CP090748:1684309-1684866 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAARLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMESALGTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231801 NZ_CP126952:4045766-4046140 [Escherichia coli O78:H4]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA