Detailed information of TA system TA023180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2944323..2944918
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GDIA_RS18745
• Protein ID: WP_012554491.1
• Coordinates: 2944323..2944505 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GDIA_RS13460
• Protein ID: WP_012554492.1
• Coordinates: 2944523..2944918 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS13430	2939824..2940006	-	183	WP_012554488.1	hypothetical protein	-
GDIA_RS13435	2940289..2940969	-	681	WP_012228581.1	TetR/AcrR family transcriptional regulator	-
GDIA_RS13440	2941153..2942754	+	1602	WP_144880527.1	efflux transporter outer membrane subunit	-
GDIA_RS13445	2942930..2943343	+	414	WP_012228583.1	RNA polymerase-binding protein DksA	-
GDIA_RS18740	2943521..2943847	-	327	WP_012228584.1	hypothetical protein	-
GDIA_RS13455	2943841..2944221	-	381	WP_043458994.1	hypothetical protein	-
GDIA_RS18745	2944323..2944505	+	183	WP_012554491.1	type II toxin-antitoxin system HicA family toxin	Toxin
GDIA_RS13460	2944523..2944918	+	396	WP_012554492.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GDIA_RS13465	2945210..2945881	-	672	Protein_2702	IS481-like element ISGdi9 family transposase	-
GDIA_RS13470	2945994..2947517	+	1524	WP_012226833.1	IS21-like element ISGdi17 family transposase	-
GDIA_RS13475	2947507..2948286	+	780	WP_012222757.1	IS21-like element ISGdi17 family helper ATPase IstB	-
GDIA_RS19280	2948551..2948760	-	210	WP_012554494.1	hypothetical protein	-
GDIA_RS13480	2949033..2949239	-	207	WP_012228610.1	cold-shock protein	-
GDIA_RS19285	2949621..2949773	+	153	WP_012554495.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2944323..2961806	17483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6642.77 Da
• Isoelectric Point: 11.7334

>T23180 WP_012554491.1 NC_011365:2944323-2944505 [Gluconacetobacter diazotrophicus PA1 5]
MNSADLIRELKKAGWMEVRSRGSHHIFRHPDHGHSVTVPHPKKGLGKGLVAAIRKQAGLK

Nucleotide

• Length: 183 bp

>T23180 NC_011365:2944323-2944505 [Gluconacetobacter diazotrophicus PA1 5]
ATGAACAGCGCCGACCTGATCCGGGAATTGAAGAAAGCGGGATGGATGGAGGTTCGGTCGCGTGGCTCTCACCACATATT
CCGCCACCCCGATCACGGACACTCGGTCACGGTGCCCCATCCCAAGAAGGGCTTGGGAAAGGGATTGGTCGCCGCCATTC
GCAAGCAGGCCGGATTGAAATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14142.76 Da
• Isoelectric Point: 4.1316

>AT23180 WP_012554492.1 NC_011365:2944523-2944918 [Gluconacetobacter diazotrophicus PA1 5]
MRYPIVIERGSDAEAYGVVVPDLPGCFSAGDTFDEAVSNASEAIALWVEDAIDNGQSIPRPSSLDGLRASLEWSGPEWIW
GFASVDPTLFDDTSERVNITLPRRVLARLDSRAKDAGETRSGFIAKLALSA

Nucleotide

• Length: 396 bp

>AT23180 NC_011365:2944523-2944918 [Gluconacetobacter diazotrophicus PA1 5]
ATGCGATATCCTATCGTGATCGAGCGCGGTTCGGACGCTGAGGCATATGGCGTTGTCGTGCCTGATTTGCCGGGGTGCTT
CTCCGCCGGCGACACGTTCGACGAGGCGGTGAGCAATGCTTCCGAGGCCATCGCTCTATGGGTCGAAGATGCCATCGATA
ACGGGCAGTCCATCCCGCGTCCGTCGTCGCTCGATGGCCTGCGCGCCAGCCTGGAATGGTCTGGACCGGAATGGATCTGG
GGCTTTGCGAGCGTCGATCCTACGTTGTTCGACGATACGTCCGAGCGTGTGAACATCACACTTCCGCGTCGTGTCCTGGC
TCGGCTGGACAGCCGGGCAAAAGATGCGGGAGAAACACGCTCTGGGTTTATCGCCAAGCTGGCGCTAAGCGCATGA