Detailed information of TA system TA023179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2622083..2622753
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A9H4U8
• Locus tag: GDIA_RS11945
• Protein ID: WP_012222568.1
• Coordinates: 2622083..2622478 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GDIA_RS11950
• Protein ID: WP_012554274.1
• Coordinates: 2622493..2622753 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS11920	2618562..2619425	+	864	WP_012222559.1	hypothetical protein	-
GDIA_RS18650	2620592..2620762	-	171	Protein_2384	IS5/IS1182 family transposase	-
GDIA_RS11935	2620874..2621338	+	465	WP_012554272.1	retroviral-like aspartic protease family protein	-
GDIA_RS11940	2621349..2622086	-	738	WP_012222567.1	ethanolamine ammonia-lyase subunit EutC	-
GDIA_RS11945	2622083..2622478	-	396	WP_012222568.1	type II toxin-antitoxin system VapC family toxin	Toxin
GDIA_RS11950	2622493..2622753	-	261	WP_012554274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GDIA_RS11955	2622867..2624105	-	1239	WP_012222572.1	alpha-hydroxy-acid oxidizing protein	-
GDIA_RS11960	2624204..2625583	-	1380	WP_012554275.1	ethanolamine ammonia-lyase subunit EutB	-
GDIA_RS11965	2626018..2627520	+	1503	WP_012222576.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (10-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14395.46 Da
• Isoelectric Point: 6.0800

>T23179 WP_012222568.1 NC_011365:c2622478-2622083 [Gluconacetobacter diazotrophicus PA1 5]
MTRMLDTNTVSHILKGHPRALERLSQIPMASVCISVITEAELRFGIAKRPEATRLHRVVNEFLQRVDVQPWESSTADSYG
NLRAALEASGKIVGSLDLLIAAHALDLNSILVTNDAGFSKIAQLETEDWTA

Nucleotide

• Length: 396 bp

>T23179 NC_011365:c2622478-2622083 [Gluconacetobacter diazotrophicus PA1 5]
ATGACCCGGATGCTCGACACCAATACGGTCAGTCACATTCTCAAGGGGCATCCGCGTGCGCTGGAGCGCCTGTCTCAGAT
CCCCATGGCGAGCGTCTGCATCTCCGTCATCACGGAAGCCGAACTGCGTTTCGGTATCGCCAAACGTCCGGAGGCGACCC
GGCTCCATCGGGTCGTGAACGAATTCCTTCAGCGCGTGGATGTCCAGCCATGGGAATCATCGACGGCCGATTCATATGGC
AACCTGCGGGCCGCGCTGGAGGCATCCGGAAAGATCGTGGGAAGTCTTGACCTCCTGATCGCCGCGCATGCCCTGGATCT
GAATTCGATCCTCGTTACGAACGACGCGGGATTCAGCAAAATCGCCCAATTGGAAACCGAGGACTGGACAGCATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9665.66 Da
• Isoelectric Point: 4.6437

>AT23179 WP_012554274.1 NC_011365:c2622753-2622493 [Gluconacetobacter diazotrophicus PA1 5]
MTQVAKIFNNGRSQAVRLPAAFRFEGTEVFIRRDEATGDVILSRRPESWDGLFAAIREGGVPDDFLSDSERDQAQDHRDP
FAEGPK

Nucleotide

• Length: 261 bp

>AT23179 NC_011365:c2622753-2622493 [Gluconacetobacter diazotrophicus PA1 5]
ATGACGCAGGTCGCAAAAATCTTCAACAATGGCCGCAGCCAGGCGGTTCGTCTGCCGGCCGCCTTTCGCTTTGAAGGCAC
GGAAGTCTTCATTCGCCGTGACGAGGCAACGGGCGACGTCATCCTTTCGCGCCGACCGGAAAGCTGGGACGGCTTGTTCG
CTGCCATCAGGGAGGGCGGCGTGCCGGACGATTTCCTCAGCGACTCCGAACGCGATCAGGCACAGGACCATCGCGATCCG
TTCGCCGAGGGACCGAAGTGA

Structures

Toxin

• AlphaFold DB: A9H4U8