Detailed information of TA system TA231789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1727551..1728821
• Replicon: chromosome
• Accession: NZ_CP090745
• Organism: Burkholderia multivorans strain 2010Ycf576V

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z34_RS10015
• Protein ID: WP_006410613.1
• Coordinates: 1728243..1728821 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BS12
• Locus tag: L0Z34_RS10010
• Protein ID: WP_006406126.1
• Coordinates: 1727551..1728108 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z34_RS09990 (L0Z34_09990)	1722847..1723872	+	1026	WP_006406131.1	aldo/keto reductase	-
L0Z34_RS09995 (L0Z34_09995)	1723972..1724415	+	444	WP_223852633.1	antibiotic biosynthesis monooxygenase	-
L0Z34_RS10000 (L0Z34_10000)	1725004..1725624	+	621	WP_006410617.1	isochorismatase family cysteine hydrolase	-
L0Z34_RS10005 (L0Z34_10005)	1725635..1727038	-	1404	WP_006406128.1	hypothetical protein	-
L0Z34_RS10010 (L0Z34_10010)	1727551..1728108	+	558	WP_006406126.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z34_RS10015 (L0Z34_10015)	1728243..1728821	+	579	WP_006410613.1	HD domain-containing protein	Toxin
L0Z34_RS10020 (L0Z34_10020)	1728922..1730460	-	1539	WP_006410596.1	MFS transporter	-
L0Z34_RS10025 (L0Z34_10025)	1730639..1731559	+	921	WP_006406123.1	LysR family transcriptional regulator	-
L0Z34_RS10030 (L0Z34_10030)	1731606..1732745	-	1140	WP_006410588.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z34_RS10035 (L0Z34_10035)	1732742..1733533	-	792	WP_035947285.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21384.10 Da
• Isoelectric Point: 4.9434

>T231789 WP_006410613.1 NZ_CP090745:1728243-1728821 [Burkholderia multivorans]
MNPDTIRARLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
APEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDAAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAQT

Nucleotide

• Length: 579 bp

>T231789 NZ_CP126952:1903307-1903558 [Escherichia coli O78:H4]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20378.30 Da
• Isoelectric Point: 7.8341

>AT231789 WP_006406126.1 NZ_CP090745:1727551-1728108 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAAHLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMETALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231789 NZ_CP126952:1903555-1903809 [Escherichia coli O78:H4]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: B9BS12