Detailed information of TA system TA231780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 748577..749232
• Replicon: chromosome
• Accession: NZ_CP090742
• Organism: Burkholderia multivorans strain 2010Ycf635

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z21_RS21770
• Protein ID: WP_006403072.1
• Coordinates: 748813..749232 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: L0Z21_RS21765
• Protein ID: WP_006403071.1
• Coordinates: 748577..748816 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z21_RS21740 (L0Z21_21740)	743639..743782	-	144	WP_006409932.1	hypothetical protein	-
L0Z21_RS21745 (L0Z21_21745)	744371..744574	+	204	WP_006403067.1	hypothetical protein	-
L0Z21_RS21750 (L0Z21_21750)	744588..745271	-	684	WP_069220955.1	Fe2+-dependent dioxygenase	-
L0Z21_RS21755 (L0Z21_21755)	745268..746023	-	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z21_RS21760 (L0Z21_21760)	746027..748273	-	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z21_RS21765 (L0Z21_21765)	748577..748816	+	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
L0Z21_RS21770 (L0Z21_21770)	748813..749232	+	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z21_RS21775 (L0Z21_21775)	749366..750661	-	1296	WP_069220956.1	aspartate carbamoyltransferase	-
L0Z21_RS21780 (L0Z21_21780)	750894..751028	+	135	WP_006409923.1	entericidin A/B family lipoprotein	-
L0Z21_RS21785 (L0Z21_21785)	751122..751697	-	576	WP_069220957.1	permease	-
L0Z21_RS21790 (L0Z21_21790)	751901..752851	-	951	WP_081336848.1	DMT family transporter	-
L0Z21_RS21795 (L0Z21_21795)	753108..753902	-	795	WP_059938960.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231780 WP_006403072.1 NZ_CP090742:748813-749232 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231780 NZ_CP090742:748813-749232 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCCTCATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT231780 WP_006403071.1 NZ_CP090742:748577-748816 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT231780 NZ_CP090742:748577-748816 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTACTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCCAACCGATCCGATCAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6