Detailed information of TA system TA231779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 586160..587430
• Replicon: chromosome
• Accession: NZ_CP090742
• Organism: Burkholderia multivorans strain 2010Ycf635

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z21_RS21075
• Protein ID: WP_065496152.1
• Coordinates: 586160..586738 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BS12
• Locus tag: L0Z21_RS21080
• Protein ID: WP_006406126.1
• Coordinates: 586873..587430 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z21_RS21055 (L0Z21_21055)	581448..582239	+	792	WP_065496148.1	enoyl-CoA hydratase	-
L0Z21_RS21060 (L0Z21_21060)	582236..583375	+	1140	WP_065496150.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z21_RS21065 (L0Z21_21065)	583422..584342	-	921	WP_065496151.1	LysR family transcriptional regulator	-
L0Z21_RS21070 (L0Z21_21070)	584521..586059	+	1539	WP_006410596.1	MFS transporter	-
L0Z21_RS21075 (L0Z21_21075)	586160..586738	-	579	WP_065496152.1	HD domain-containing protein	Toxin
L0Z21_RS21080 (L0Z21_21080)	586873..587430	-	558	WP_006406126.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z21_RS21085 (L0Z21_21085)	587943..589346	+	1404	WP_006406128.1	hypothetical protein	-
L0Z21_RS21090 (L0Z21_21090)	589357..589881	-	525	WP_105774904.1	isochorismatase family cysteine hydrolase	-
L0Z21_RS21095 (L0Z21_21095)	590568..591011	-	444	WP_223852633.1	antibiotic biosynthesis monooxygenase	-
L0Z21_RS21100 (L0Z21_21100)	591111..592136	-	1026	WP_006406131.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21397.10 Da
• Isoelectric Point: 5.1007

>T231779 WP_065496152.1 NZ_CP090742:c586738-586160 [Burkholderia multivorans]
MNPDTIRARLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
ATEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDAAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAHT

Nucleotide

• Length: 579 bp

>T231779 NZ_CP126951:17397-17918 [Escherichia coli O78:H4]
GTGAGCAATACGACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGTCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACACGCTATGCGTGTTGTGTATAATGCCTCTCTTGCTGTTGGTATTCATGGACTCTTCGTTG
AGGCGTTGAATGATAAAGCTAAGGCGTTTTATAAAAGTCTGGGCTTTATCCAGTTGATTGGAAACAACGAGCGATCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20378.30 Da
• Isoelectric Point: 7.8341

>AT231779 WP_006406126.1 NZ_CP090742:c587430-586873 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAAHLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMETALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231779 NZ_CP126951:17120-17389 [Escherichia coli O78:H4]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATAACCCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTAAAGGTTATAGAACAACATCGAAGAC
TGGTTCTGAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGATAAGCTGAAACGA
GCTGCTAATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: B9BS12