Detailed information of TA system TA231775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2436717..2438239
• Replicon: chromosome
• Accession: NZ_CP090740
• Organism: Burkholderia multivorans strain 2010Ycf635

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: L0Z21_RS11495
• Protein ID: WP_019397543.1
• Coordinates: 2437313..2438239 (+)
• Length: 309 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: L0Z21_RS11490
• Protein ID: WP_217078439.1
• Coordinates: 2436717..2437316 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z21_RS11470 (L0Z21_11470)	2433733..2434401	+	669	WP_006400736.1	OmpA family protein	-
L0Z21_RS11475 (L0Z21_11475)	2434556..2435251	+	696	WP_249487614.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
L0Z21_RS11480 (L0Z21_11480)	2435265..2435981	+	717	WP_012213845.1	phosphoglycolate phosphatase	-
L0Z21_RS11490 (L0Z21_11490)	2436717..2437316	+	600	WP_217078439.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
L0Z21_RS11495 (L0Z21_11495)	2437313..2438239	+	927	WP_019397543.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
L0Z21_RS11500 (L0Z21_11500)	2438236..2438727	+	492	WP_217078432.1	hypothetical protein	-
L0Z21_RS11505 (L0Z21_11505)	2438724..2439311	+	588	WP_217078431.1	hypothetical protein	-
L0Z21_RS11510 (L0Z21_11510)	2439343..2439774	-	432	WP_217078430.1	LacI family transcriptional regulator	-
L0Z21_RS11515 (L0Z21_11515)	2439771..2441126	-	1356	WP_249460597.1	recombinase family protein	-
L0Z21_RS11520 (L0Z21_11520)	2441123..2441623	-	501	WP_217078770.1	DUF2924 domain-containing protein	-
L0Z21_RS11525 (L0Z21_11525)	2441620..2441838	-	219	WP_217078769.1	hypothetical protein	-
L0Z21_RS11530 (L0Z21_11530)	2441964..2443178	-	1215	WP_217078768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2436717..2448731	12014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-257)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (7-53)

Sequences

Toxin

Protein

• Length: 309 a.a.
• Molecular weight: 34471.36 Da
• Isoelectric Point: 6.7011

>T231775 WP_019397543.1 NZ_CP090740:2437313-2438239 [Burkholderia multivorans]
VSKRNTAASVRARLLVKARTDKQDFNLVLTRYALERLLYRLSVSAHADHFLLKGALLFDLWFDIPHRPTRDADLLGFGSA
EIPHVEAAFREICAVELDDGIRFQADSVHAEEIRKEANYSGVRVTLLGLLDGARCHVQVDVGFGDAVTPGPEAVDYPVML
PDMPAPKLRAYPRYTVVAEKLEALVSLGIANSRMKDYFDLWILSRHSEFDGALLSKAIHATFERRRTPLPDGVPFGLSDE
FAQDRQKQTQWQAFLRKNALVELQLSEVIAGLRAFLSFPLDALRQAAAFPLAWRADSGWTPSTEESGR

Nucleotide

• Length: 927 bp

>T231775 NZ_CP126948:c4526371-4526198 [Escherichia coli O78:H4]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22296.91 Da
• Isoelectric Point: 9.9667

>AT231775 WP_217078439.1 NZ_CP090740:2436717-2437316 [Burkholderia multivorans]
MDSSHQSILELAAQRGLIRPRDLNERGLPTVALTRLVRQGLLQRVGRGLYAIPDRPVSEHGALAEVARKNPQAIVCLLSA
LRVHELTTQSPFEVWLAIPNKARAPKMDYPPLRIVRFSGAALTEGIEEHSVDGVPVRVTNVARTVADCFKFRNKIGLDVA
LEALQEAWRAKRVSMDELWRFAELCRVANVMRPYLESLT

Nucleotide

• Length: 600 bp

>AT231775 NZ_CP126948:c4526695-4526399 [Escherichia coli O78:H4]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG