Detailed information of TA system TA231774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 1030315..1030997
• Replicon: chromosome
• Accession: NZ_CP090740
• Organism: Burkholderia multivorans strain 2010Ycf635

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: L0Z21_RS04970
• Protein ID: WP_069220063.1
• Coordinates: 1030638..1030997 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: L0Z21_RS04965
• Protein ID: WP_006405635.1
• Coordinates: 1030315..1030638 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z21_RS04945 (L0Z21_04945)	1025664..1027124	+	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-
L0Z21_RS04950 (L0Z21_04950)	1027283..1029181	+	1899	WP_069220062.1	molecular chaperone HtpG	-
L0Z21_RS04955 (L0Z21_04955)	1029190..1029804	+	615	WP_006398690.1	chorismate lyase	-
L0Z21_RS04960 (L0Z21_04960)	1029801..1030292	+	492	WP_053296985.1	DNA-deoxyinosine glycosylase	-
L0Z21_RS04965 (L0Z21_04965)	1030315..1030638	-	324	WP_006405635.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z21_RS04970 (L0Z21_04970)	1030638..1030997	-	360	WP_069220063.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z21_RS04975 (L0Z21_04975)	1031545..1031799	-	255	WP_059940739.1	hypothetical protein	-
L0Z21_RS04980 (L0Z21_04980)	1032028..1032945	+	918	WP_053296988.1	spermidine synthase	-
L0Z21_RS04985 (L0Z21_04985)	1033142..1033375	+	234	WP_006398697.1	DUF3311 domain-containing protein	-
L0Z21_RS04990 (L0Z21_04990)	1033372..1034922	+	1551	WP_006398698.1	sodium:solute symporter family protein	-
L0Z21_RS04995 (L0Z21_04995)	1035026..1035472	-	447	WP_006405630.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12917.82 Da
• Isoelectric Point: 9.3081

>T231774 WP_069220063.1 NZ_CP090740:c1030997-1030638 [Burkholderia multivorans]
MVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTVKLGGAV
YVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGEK

Nucleotide

• Length: 360 bp

>T231774 NZ_CP126948:4514861-4515235 [Escherichia coli O78:H4]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT231774 WP_006405635.1 NZ_CP090740:c1030638-1030315 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT231774 NZ_CP126948:4514403-4514771 [Escherichia coli O78:H4]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCACCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: B9BQB8