Detailed information of TA system TA231773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2664877..2665497
• Replicon: chromosome
• Accession: NZ_CP090738
• Organism: Burkholderia cepacia strain 2011Ycf427V

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L0Z22_RS28785
• Protein ID: WP_021157289.1
• Coordinates: 2664877..2665194 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: L0Z22_RS28790
• Protein ID: WP_021157290.1
• Coordinates: 2665198..2665497 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z22_RS28775 (L0Z22_28775)	2661413..2662846	-	1434	WP_021157287.1	aldehyde dehydrogenase family protein	-
L0Z22_RS28780 (L0Z22_28780)	2662876..2664516	-	1641	WP_021157288.1	acetolactate synthase large subunit	-
L0Z22_RS28785 (L0Z22_28785)	2664877..2665194	+	318	WP_021157289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z22_RS28790 (L0Z22_28790)	2665198..2665497	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
L0Z22_RS28795 (L0Z22_28795)	2665554..2666840	-	1287	WP_060343380.1	DUF445 domain-containing protein	-
L0Z22_RS28800 (L0Z22_28800)	2667052..2668272	-	1221	WP_249453804.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
L0Z22_RS28805 (L0Z22_28805)	2668262..2668588	-	327	WP_048022186.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
L0Z22_RS28810 (L0Z22_28810)	2668609..2669094	-	486	WP_048022184.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
L0Z22_RS28815 (L0Z22_28815)	2669112..2670383	-	1272	WP_048022287.1	anthranilate 1,2-dioxygenase large subunit AndAc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-91)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11866.62 Da
• Isoelectric Point: 9.9423

>T231773 WP_021157289.1 NZ_CP090738:2664877-2665194 [Burkholderia cepacia]
MPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVILL
CGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T231773 NZ_CP126948:3960847-3961224 [Escherichia coli O78:H4]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT231773 WP_021157290.1 NZ_CP090738:2665198-2665497 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT231773 NZ_CP126948:3960426-3960800 [Escherichia coli O78:H4]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: U2GAF3