Detailed information of TA system TA231771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2240858..2241435
• Replicon: chromosome
• Accession: NZ_CP090738
• Organism: Burkholderia cepacia strain 2011Ycf427V

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8I1DRR3
• Locus tag: L0Z22_RS26895
• Protein ID: WP_048026025.1
• Coordinates: 2241070..2241435 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B2Q139
• Locus tag: L0Z22_RS26890
• Protein ID: WP_048026026.1
• Coordinates: 2240858..2241076 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z22_RS26865 (L0Z22_26865)	2236072..2236554	+	483	WP_249453701.1	GNAT family N-acetyltransferase	-
L0Z22_RS26870 (L0Z22_26870)	2236650..2237915	-	1266	WP_048026028.1	Zn-dependent hydrolase	-
L0Z22_RS26875 (L0Z22_26875)	2237908..2238432	-	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z22_RS26880 (L0Z22_26880)	2238492..2239658	-	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z22_RS26885 (L0Z22_26885)	2239836..2240732	+	897	WP_048026027.1	LysR substrate-binding domain-containing protein	-
L0Z22_RS26890 (L0Z22_26890)	2240858..2241076	+	219	WP_048026026.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z22_RS26895 (L0Z22_26895)	2241070..2241435	+	366	WP_048026025.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z22_RS26900 (L0Z22_26900)	2241801..2242364	+	564	WP_006488685.1	alkyl hydroperoxide reductase subunit C	-
L0Z22_RS26905 (L0Z22_26905)	2242493..2244091	+	1599	WP_182670402.1	alkyl hydroperoxide reductase subunit F	-
L0Z22_RS26910 (L0Z22_26910)	2244270..2244824	-	555	WP_230945234.1	DUF4276 family protein	-
L0Z22_RS26915 (L0Z22_26915)	2244860..2246047	-	1188	WP_081057995.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13610.67 Da
• Isoelectric Point: 8.4826

>T231771 WP_048026025.1 NZ_CP090738:2241070-2241435 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARVELARYSYRAISIISWMEVLVGTSAHDENTTRAWLSSFDVIALDSTIANRAVTIRQER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGIRMPYRI

Nucleotide

• Length: 366 bp

>T231771 NZ_CP126948:c3592304-3592002 [Escherichia coli O78:H4]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8257.35 Da
• Isoelectric Point: 4.4419

>AT231771 WP_048026026.1 NZ_CP090738:2240858-2241076 [Burkholderia cepacia]
MSRILVDLSDGQLDELAAIVETERRPRAAIIRDAIDAYIALHKRPLADDAFGLWKDRTVDGLAYQEEMRSEW

Nucleotide

• Length: 219 bp

>AT231771 NZ_CP126948:c3591966-3591625 [Escherichia coli O78:H4]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A8B2Q139