Detailed information of TA system TA023177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 1917411..1917933
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: parE
• Uniprot ID: B5ZKX9
• Locus tag: GDIA_RS08705
• Protein ID: WP_012553951.1
• Coordinates: 1917411..1917701 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GDIA_RS08710
• Protein ID: WP_211205851.1
• Coordinates: 1917694..1917933 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS18545	1913525..1914582	+	1058	WP_157864088.1	IS630-like element ISGdi4 family transposase	-
GDIA_RS08695	1914840..1915265	-	426	WP_012553949.1	hypothetical protein	-
GDIA_RS08700	1915271..1917253	-	1983	WP_012553950.1	conjugal transfer protein TraG	-
GDIA_RS08705	1917411..1917701	-	291	WP_012553951.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GDIA_RS08710	1917694..1917933	-	240	WP_211205851.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GDIA_RS08715	1918039..1918902	-	864	WP_012553953.1	LysR family transcriptional regulator	-
GDIA_RS08720	1918978..1919589	+	612	Protein_1752	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GDIA_RS08725	1919627..1920877	+	1251	WP_012553139.1	IS701-like element ISGdi12 family transposase	-
GDIA_RS08730	1920993..1921397	+	405	Protein_1754	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GDIA_RS08735	1921394..1922074	+	681	WP_012553954.1	hypothetical protein	-
GDIA_RS08740	1922089..1922451	+	363	WP_012553955.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1818917..1935901	116984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-91)

Antitoxin

• Antitoxin ParD (11-60)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11109.61 Da
• Isoelectric Point: 6.9876

>T23177 WP_012553951.1 NC_011365:c1917701-1917411 [Gluconacetobacter diazotrophicus PA1 5]
MPSYTLSGEADSDIQAIIALSIRNWGWARAERYILDLHAAFETLATYPDMGADIGDLRPGYFRFVHDSHAVFYRKAGERI
FVVRVLHQRQEPKHYL

Nucleotide

• Length: 291 bp

>T23177 NC_011365:c1917701-1917411 [Gluconacetobacter diazotrophicus PA1 5]
ATGCCTAGCTACACGCTTTCCGGCGAAGCGGATTCCGACATTCAGGCCATCATTGCTCTCTCGATCCGGAACTGGGGATG
GGCGCGCGCCGAGCGCTATATCCTCGACCTGCACGCGGCCTTCGAGACGCTGGCGACCTATCCTGACATGGGAGCAGATA
TCGGCGACCTGCGGCCCGGCTATTTCCGCTTCGTCCATGACAGCCATGCCGTTTTCTATCGGAAGGCGGGAGAGCGGATT
TTCGTCGTTCGTGTCCTGCACCAGCGCCAAGAGCCGAAACACTACCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8845.90 Da
• Isoelectric Point: 4.7265

>AT23177 WP_211205851.1 NC_011365:c1917933-1917694 [Gluconacetobacter diazotrophicus PA1 5]
MNVSVPDPMRDWVQRRIDSGQYASVSDYVRDLIRRDQTQAEERQALVEALVQGERSGVSKRTIPDILAAMKTAPDATDA

Nucleotide

• Length: 240 bp

>AT23177 NC_011365:c1917933-1917694 [Gluconacetobacter diazotrophicus PA1 5]
ATGAACGTCTCCGTGCCGGACCCGATGCGCGACTGGGTACAGCGCCGTATCGACAGCGGGCAATATGCCAGCGTGAGCGA
CTATGTACGTGACCTGATCAGGCGCGACCAGACACAGGCCGAGGAACGGCAGGCTCTGGTCGAGGCCCTGGTCCAGGGCG
AACGGAGCGGTGTGAGCAAACGCACAATCCCCGATATCCTCGCGGCGATGAAGACGGCCCCCGACGCGACGGATGCCTAG

Structures

Toxin

• AlphaFold DB: B5ZKX9