Detailed information of TA system TA231769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1576356..1576995
• Replicon: chromosome
• Accession: NZ_CP090738
• Organism: Burkholderia cepacia strain 2011Ycf427V

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z22_RS24030
• Protein ID: WP_230962748.1
• Coordinates: 1576579..1576995 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2G525
• Locus tag: L0Z22_RS24025
• Protein ID: WP_021159426.1
• Coordinates: 1576356..1576592 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z22_RS24005 (L0Z22_24005)	1571458..1572231	+	774	WP_060341252.1	SDR family oxidoreductase	-
L0Z22_RS24010 (L0Z22_24010)	1572302..1573537	-	1236	WP_060341253.1	acyltransferase	-
L0Z22_RS24015 (L0Z22_24015)	1573834..1574577	-	744	WP_249455293.1	methyltransferase domain-containing protein	-
L0Z22_RS24020 (L0Z22_24020)	1575122..1575959	+	838	Protein_1390	hypothetical protein	-
L0Z22_RS24025 (L0Z22_24025)	1576356..1576592	+	237	WP_021159426.1	hypothetical protein	Antitoxin
L0Z22_RS24030 (L0Z22_24030)	1576579..1576995	+	417	WP_230962748.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z22_RS24035 (L0Z22_24035)	1577195..1578091	+	897	WP_048025080.1	VOC family protein	-
L0Z22_RS24040 (L0Z22_24040)	1578101..1578994	+	894	WP_042975012.1	CoA-transferase	-
L0Z22_RS24045 (L0Z22_24045)	1579005..1579802	+	798	WP_048025082.1	ketoacid CoA transferase	-
L0Z22_RS24050 (L0Z22_24050)	1579811..1580731	+	921	WP_249455294.1	enoyl-CoA hydratase	-
L0Z22_RS24055 (L0Z22_24055)	1580728..1581828	+	1101	WP_059707397.1	nitronate monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15278.57 Da
• Isoelectric Point: 8.5855

>T231769 WP_230962748.1 NZ_CP090738:1576579-1576995 [Burkholderia cepacia]
VYLIDTNVISEIRKGKRTNRGVRAFFRQAAADASPLYLSVVTVAELRRGVDLIRHRGDHPQASALEAWVAMILAGYAPNI
LPVDIEISQTWGHLRVPDPTHELDKLIAATALINDLTVVTRNVADFARTGVRLLNPFD

Nucleotide

• Length: 417 bp

>T231769 NZ_CP126948:2953791-2954285 [Escherichia coli O78:H4]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGATCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8681.75 Da
• Isoelectric Point: 5.8214

>AT231769 WP_021159426.1 NZ_CP090738:1576356-1576592 [Burkholderia cepacia]
MANLLVRNVDDNIVQSLRERAAANGRSAEAEHRAILADALGRPKRKTFAQVLMSMPDVGEDADFQRIQDSGEARRVFD

Nucleotide

• Length: 237 bp

>AT231769 NZ_CP126948:2953501-2953794 [Escherichia coli O78:H4]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: U2G525