Detailed information of TA system TA231765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 348244..348809
• Replicon: chromosome
• Accession: NZ_CP090738
• Organism: Burkholderia cepacia strain 2011Ycf427V

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E1S7L3
• Locus tag: L0Z22_RS18655
• Protein ID: WP_060343311.1
• Coordinates: 348244..348615 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1FGI9
• Locus tag: L0Z22_RS18660
• Protein ID: WP_006752328.1
• Coordinates: 348612..348809 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z22_RS18640 (L0Z22_18640)	344107..344832	+	726	WP_021159298.1	site-2 protease family protein	-
L0Z22_RS18645 (L0Z22_18645)	344895..346274	-	1380	WP_059591042.1	MFS transporter	-
L0Z22_RS18650 (L0Z22_18650)	346520..348097	+	1578	WP_249454468.1	aldehyde dehydrogenase (NADP(+))	-
L0Z22_RS18655 (L0Z22_18655)	348244..348615	-	372	WP_060343311.1	PIN domain-containing protein	Toxin
L0Z22_RS18660 (L0Z22_18660)	348612..348809	-	198	WP_006752328.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z22_RS18665 (L0Z22_18665)	349058..349609	+	552	WP_048023383.1	hypothetical protein	-
L0Z22_RS18670 (L0Z22_18670)	349793..350707	-	915	WP_048023382.1	5-dehydro-4-deoxyglucarate dehydratase	-
L0Z22_RS18675 (L0Z22_18675)	350936..352489	+	1554	WP_249454469.1	galactarate dehydratase	-
L0Z22_RS18680 (L0Z22_18680)	352556..353038	-	483	WP_042974910.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13384.42 Da
• Isoelectric Point: 5.5223

>T231765 WP_060343311.1 NZ_CP090738:c348615-348244 [Burkholderia cepacia]
MILVDTSVWIDHLRAGDATLTTLLEAERVLIHPFVVGELALGSLRDRETVLDALRDLPAAAAATDDEVQRMIDVAPLYGL
GIGYVDAHLLASVRLTSGSKLWTRDRRLLAAAERLQLAAHPAH

Nucleotide

• Length: 372 bp

>T231765 NZ_CP126948:2156540-2156642 [Escherichia coli O78:H4]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7054.10 Da
• Isoelectric Point: 10.6826

>AT231765 WP_006752328.1 NZ_CP090738:c348809-348612 [Burkholderia cepacia]
MRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSEPA

Nucleotide

• Length: 198 bp

>AT231765 NZ_CP126948:c2156680-2156412 [Escherichia coli O78:H4]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A2S9KMB8