Detailed information of TA system TA231763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3555795..3556432
• Replicon: chromosome
• Accession: NZ_CP090737
• Organism: Burkholderia cepacia strain 2011Ycf427V

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2FRJ0
• Locus tag: L0Z22_RS16685
• Protein ID: WP_021164186.1
• Coordinates: 3555795..3556196 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: L0Z22_RS16690
• Protein ID: WP_042978332.1
• Coordinates: 3556196..3556432 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z22_RS16675 (L0Z22_16675)	3553172..3554308	+	1137	WP_249452833.1	alginate lyase family protein	-
L0Z22_RS16680 (L0Z22_16680)	3554339..3555727	+	1389	WP_021164187.1	L-serine ammonia-lyase	-
L0Z22_RS16685 (L0Z22_16685)	3555795..3556196	-	402	WP_021164186.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z22_RS16690 (L0Z22_16690)	3556196..3556432	-	237	WP_042978332.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z22_RS16695 (L0Z22_16695)	3556814..3558517	+	1704	WP_249452835.1	thiamine pyrophosphate-binding protein	-
L0Z22_RS16700 (L0Z22_16700)	3558514..3559626	+	1113	WP_249452841.1	ADP-heptose--LPS heptosyltransferase	-
L0Z22_RS16705 (L0Z22_16705)	3559896..3560927	-	1032	WP_042978041.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15033.14 Da
• Isoelectric Point: 5.2050

>T231763 WP_021164186.1 NZ_CP090737:c3556196-3555795 [Burkholderia cepacia]
MPRYMLDTNMCIYLMKHQPEQVAKRFAQCYTGDVVMSAITYAELEYGVAACANAVRERRNLADLIEDIPVAPFDAAAAQA
YGPVREATRERKRDHLDKLIAAHAVSLDVVLVTNNERDFASYPGLRLENWLND

Nucleotide

• Length: 402 bp

>T231763 NZ_CP126948:1911089-1911343 [Escherichia coli O78:H4]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8926.06 Da
• Isoelectric Point: 5.1651

>AT231763 WP_042978332.1 NZ_CP090737:c3556432-3556196 [Burkholderia cepacia]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERIGDEIRIRPVRRPLAGVLEKFAKFAPDFMAEGRGDQEQADREGL

Nucleotide

• Length: 237 bp

>AT231763 NZ_CP126948:1910841-1911092 [Escherichia coli O78:H4]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: U2FRJ0