Detailed information of TA system TA023176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1808300..1808934
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GDIA_RS08155
• Protein ID: WP_012222312.1
• Coordinates: 1808300..1808710 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A9GZV6
• Locus tag: GDIA_RS08160
• Protein ID: WP_012222311.1
• Coordinates: 1808710..1808934 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS08135	1804011..1804787	+	777	WP_012553884.1	imidazole glycerol phosphate synthase subunit HisF	-
GDIA_RS08140	1804829..1805356	+	528	WP_012222315.1	phosphoribosyl-ATP diphosphatase	-
GDIA_RS08145	1805380..1805760	+	381	WP_012553885.1	histidine triad nucleotide-binding protein	-
GDIA_RS08150	1805791..1808139	+	2349	WP_012222313.1	CRISPR-associated endonuclease Cas3''	-
GDIA_RS08155	1808300..1808710	-	411	WP_012222312.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GDIA_RS08160	1808710..1808934	-	225	WP_012222311.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GDIA_RS08165	1809185..1809859	+	675	WP_211205850.1	type I-C CRISPR-associated protein Cas5	-
GDIA_RS08170	1809856..1811679	+	1824	WP_012553887.1	type I-C CRISPR-associated protein Cas8c/Csd1	-
GDIA_RS08175	1811672..1812610	+	939	WP_012553888.1	type I-C CRISPR-associated protein Cas7/Csd2	-
GDIA_RS08180	1812619..1813275	+	657	WP_012222307.1	CRISPR-associated protein Cas4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15419.60 Da
• Isoelectric Point: 6.7075

>T23176 WP_012222312.1 NC_011365:c1808710-1808300 [Gluconacetobacter diazotrophicus PA1 5]
MLRFLLDTNLCIRVLRDRPQGLRARFNAYAEDLCISDVVLYELLYGAERSSDPVRVRREVEHFADRLNVLPFDSAAASHT
AEIRDDLERRGCVIGPYDLMIAGHARSRGLVVVTGNLGEFRRVAGLRAEDWFTNVA

Nucleotide

• Length: 411 bp

>T23176 NC_011365:c1808710-1808300 [Gluconacetobacter diazotrophicus PA1 5]
ATGCTGCGCTTTCTGCTGGACACCAACTTGTGCATCCGTGTGTTGCGGGACCGGCCGCAGGGCCTGCGGGCGCGGTTCAA
TGCCTACGCCGAAGACCTCTGTATCTCCGATGTCGTGCTATACGAACTGCTCTATGGGGCGGAGCGGTCGTCCGATCCCG
TGCGTGTCAGGCGAGAGGTCGAGCATTTCGCGGACAGACTGAATGTCCTGCCGTTCGACAGCGCGGCGGCGTCCCATACG
GCCGAGATCCGGGACGATCTCGAACGCAGGGGCTGCGTGATCGGCCCCTATGATCTGATGATCGCGGGACACGCCCGCAG
CCGGGGGTTGGTGGTCGTCACAGGCAATCTGGGCGAGTTCAGGCGCGTTGCGGGGCTCAGGGCCGAAGACTGGTTCACGA
ATGTTGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8240.46 Da
• Isoelectric Point: 6.2937

>AT23176 WP_012222311.1 NC_011365:c1808934-1808710 [Gluconacetobacter diazotrophicus PA1 5]
MLTRTTLFLSNRSQAVRLPKAVAFGEGVREVVIVPEGPRRIIAPAEAAWDDFFAAPGLDLGERSQPAMQERERF

Nucleotide

• Length: 225 bp

>AT23176 NC_011365:c1808934-1808710 [Gluconacetobacter diazotrophicus PA1 5]
ATGCTCACGCGCACGACCTTGTTTCTCTCCAATCGTTCCCAGGCGGTTCGCCTGCCGAAGGCCGTGGCCTTTGGAGAAGG
CGTGCGGGAGGTGGTGATTGTTCCCGAAGGCCCGCGTCGGATTATCGCGCCCGCTGAAGCAGCCTGGGACGATTTTTTCG
CCGCTCCCGGCCTGGACCTGGGGGAACGCAGCCAGCCGGCGATGCAGGAACGGGAGCGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A9GZV6