Detailed information of TA system TA231757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 722640..723295
• Replicon: chromosome
• Accession: NZ_CP090735
• Organism: Burkholderia multivorans strain 2011Ycf521III

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z43_RS21495
• Protein ID: WP_006403072.1
• Coordinates: 722876..723295 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: L0Z43_RS21490
• Protein ID: WP_006403071.1
• Coordinates: 722640..722879 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z43_RS21465 (L0Z43_21465)	717702..717845	-	144	WP_006409932.1	hypothetical protein	-
L0Z43_RS21470 (L0Z43_21470)	718434..718637	+	204	WP_006403067.1	hypothetical protein	-
L0Z43_RS21475 (L0Z43_21475)	718651..719334	-	684	WP_069220955.1	Fe2+-dependent dioxygenase	-
L0Z43_RS21480 (L0Z43_21480)	719331..720086	-	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z43_RS21485 (L0Z43_21485)	720090..722336	-	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z43_RS21490 (L0Z43_21490)	722640..722879	+	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
L0Z43_RS21495 (L0Z43_21495)	722876..723295	+	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z43_RS21500 (L0Z43_21500)	723429..724724	-	1296	WP_069220956.1	aspartate carbamoyltransferase	-
L0Z43_RS21505 (L0Z43_21505)	724957..725091	+	135	WP_006409923.1	entericidin A/B family lipoprotein	-
L0Z43_RS21510 (L0Z43_21510)	725185..725760	-	576	WP_069220957.1	permease	-
L0Z43_RS21515 (L0Z43_21515)	725964..726914	-	951	WP_081336848.1	DMT family transporter	-
L0Z43_RS21520 (L0Z43_21520)	727171..727965	-	795	WP_059938960.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231757 WP_006403072.1 NZ_CP090735:722876-723295 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231757 NZ_CP126948:293950-294327 [Escherichia coli O78:H4]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT231757 WP_006403071.1 NZ_CP090735:722640-722879 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT231757 NZ_CP126948:293732-293953 [Escherichia coli O78:H4]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6