Detailed information of TA system TA231750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2676190..2676810
• Replicon: chromosome
• Accession: NZ_CP090732
• Organism: Burkholderia cepacia strain 2011Ycf793I

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L0Z05_RS34915
• Protein ID: WP_021157289.1
• Coordinates: 2676190..2676507 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: L0Z05_RS34920
• Protein ID: WP_021157290.1
• Coordinates: 2676511..2676810 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z05_RS34905 (L0Z05_34905)	2672726..2674159	-	1434	WP_021157287.1	aldehyde dehydrogenase family protein	-
L0Z05_RS34910 (L0Z05_34910)	2674189..2675829	-	1641	WP_021157288.1	acetolactate synthase large subunit	-
L0Z05_RS34915 (L0Z05_34915)	2676190..2676507	+	318	WP_021157289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z05_RS34920 (L0Z05_34920)	2676511..2676810	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
L0Z05_RS34925 (L0Z05_34925)	2676867..2678153	-	1287	WP_060343380.1	DUF445 domain-containing protein	-
L0Z05_RS34930 (L0Z05_34930)	2678365..2679585	-	1221	WP_249453804.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
L0Z05_RS34935 (L0Z05_34935)	2679575..2679901	-	327	WP_048022186.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
L0Z05_RS34940 (L0Z05_34940)	2679922..2680407	-	486	WP_048022184.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
L0Z05_RS34945 (L0Z05_34945)	2680425..2681696	-	1272	WP_048022287.1	anthranilate 1,2-dioxygenase large subunit AndAc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-91)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11866.62 Da
• Isoelectric Point: 9.9423

>T231750 WP_021157289.1 NZ_CP090732:2676190-2676507 [Burkholderia cepacia]
MPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVILL
CGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T231750 NZ_CP126947:c104878-104591 [Escherichia coli O78:H51]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT231750 WP_021157290.1 NZ_CP090732:2676511-2676810 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT231750 NZ_CP126947:c105126-104875 [Escherichia coli O78:H51]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3