Detailed information of TA system TA023175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1204001..1204601
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B5ZGE8
• Locus tag: GDIA_RS05295
• Protein ID: WP_012553569.1
• Coordinates: 1204257..1204601 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B5ZGE7
• Locus tag: GDIA_RS05290
• Protein ID: WP_012553568.1
• Coordinates: 1204001..1204264 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS05270	1199910..1200998	-	1089	WP_012222184.1	IS110-like element ISGdi15 family transposase	-
GDIA_RS05275	1201674..1202150	-	477	WP_043458730.1	DUF2840 domain-containing protein	-
GDIA_RS05280	1202456..1203127	-	672	WP_012553566.1	DNA methyltransferase	-
GDIA_RS05285	1203654..1203893	+	240	WP_012553567.1	hypothetical protein	-
GDIA_RS05290	1204001..1204264	+	264	WP_012553568.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GDIA_RS05295	1204257..1204601	+	345	WP_012553569.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GDIA_RS18440	1204634..1206322	+	1689	WP_012553570.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1199910..1200998	1088

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13270.05 Da
• Isoelectric Point: 9.7132

>T23175 WP_012553569.1 NC_011365:1204257-1204601 [Gluconacetobacter diazotrophicus PA1 5]
MTEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQDGYRLVYQVVEQRIVIFVIAV
GRREDNEIYTAATGRIPETPADRTHQPSGRSRKR

Nucleotide

• Length: 345 bp

>T23175 NC_011365:1204257-1204601 [Gluconacetobacter diazotrophicus PA1 5]
ATGACTGAATACGCGATCGAGTTCGACGATCGCGCTCTCCGCGAGTGGGATGGGCTGGACGGCAGCATTCGCAGGAAGTT
CGAGAAAAAGCTCGAAAAGCTTGTTCTGAACCCGCACTCTCCCGGTAACGAACTGCACGGCGATCTCGCCGGATTCTACA
AGATCAAACTGCGCCAGGACGGCTACCGCCTCGTCTATCAGGTCGTGGAACAGCGGATCGTCATCTTCGTCATCGCGGTC
GGACGCCGTGAGGACAACGAAATCTACACGGCGGCAACCGGGCGCATCCCGGAGACGCCAGCCGATCGGACACATCAGCC
CAGCGGCAGAAGCCGCAAGAGGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9638.06 Da
• Isoelectric Point: 7.2041

>AT23175 WP_012553568.1 NC_011365:1204001-1204264 [Gluconacetobacter diazotrophicus PA1 5]
MSAVTFRVDDTLKAAAVAKLSAQGMSLSDVLRDTLAYIAETGQPPVKRRLVTDEDARLIEIVRERLADPAPRHRMTLADL
KARHPDD

Nucleotide

• Length: 264 bp

>AT23175 NC_011365:1204001-1204264 [Gluconacetobacter diazotrophicus PA1 5]
ATGTCCGCCGTGACGTTCCGTGTCGATGACACCCTCAAGGCCGCCGCTGTCGCGAAGCTGTCGGCGCAAGGCATGTCCCT
GTCCGACGTGCTGCGCGATACCCTGGCCTATATCGCCGAGACCGGCCAGCCGCCGGTCAAGCGCCGCCTTGTGACCGACG
AGGATGCAAGGCTGATCGAGATCGTCCGCGAACGCCTCGCCGATCCCGCGCCGCGTCATCGCATGACGCTGGCGGACTTG
AAGGCCCGCCATCCGGATGACTGA

Structures

Toxin

• AlphaFold DB: B5ZGE8

Antitoxin

• AlphaFold DB: B5ZGE7