Detailed information of TA system TA231748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2252171..2252748
• Replicon: chromosome
• Accession: NZ_CP090732
• Organism: Burkholderia cepacia strain 2011Ycf793I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8I1DRR3
• Locus tag: L0Z05_RS33025
• Protein ID: WP_048026025.1
• Coordinates: 2252383..2252748 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B2Q139
• Locus tag: L0Z05_RS33020
• Protein ID: WP_048026026.1
• Coordinates: 2252171..2252389 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z05_RS32995 (L0Z05_32995)	2247385..2247867	+	483	WP_249453701.1	GNAT family N-acetyltransferase	-
L0Z05_RS33000 (L0Z05_33000)	2247963..2249228	-	1266	WP_048026028.1	Zn-dependent hydrolase	-
L0Z05_RS33005 (L0Z05_33005)	2249221..2249745	-	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z05_RS33010 (L0Z05_33010)	2249805..2250971	-	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z05_RS33015 (L0Z05_33015)	2251149..2252045	+	897	WP_048026027.1	LysR substrate-binding domain-containing protein	-
L0Z05_RS33020 (L0Z05_33020)	2252171..2252389	+	219	WP_048026026.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z05_RS33025 (L0Z05_33025)	2252383..2252748	+	366	WP_048026025.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z05_RS33030 (L0Z05_33030)	2253114..2253677	+	564	WP_006488685.1	alkyl hydroperoxide reductase subunit C	-
L0Z05_RS33035 (L0Z05_33035)	2253806..2255404	+	1599	WP_182670402.1	alkyl hydroperoxide reductase subunit F	-
L0Z05_RS33040 (L0Z05_33040)	2255583..2256137	-	555	WP_230945234.1	DUF4276 family protein	-
L0Z05_RS33045 (L0Z05_33045)	2256173..2257360	-	1188	WP_081057995.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13610.67 Da
• Isoelectric Point: 8.4826

>T231748 WP_048026025.1 NZ_CP090732:2252383-2252748 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARVELARYSYRAISIISWMEVLVGTSAHDENTTRAWLSSFDVIALDSTIANRAVTIRQER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGIRMPYRI

Nucleotide

• Length: 366 bp

>T231748 NZ_CP126947:c78538-78260 [Escherichia coli O78:H51]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8257.35 Da
• Isoelectric Point: 4.4419

>AT231748 WP_048026026.1 NZ_CP090732:2252171-2252389 [Burkholderia cepacia]
MSRILVDLSDGQLDELAAIVETERRPRAAIIRDAIDAYIALHKRPLADDAFGLWKDRTVDGLAYQEEMRSEW

Nucleotide

• Length: 219 bp

>AT231748 NZ_CP126947:c78807-78538 [Escherichia coli O78:H51]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B2Q139