Detailed information of TA system TA231746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1587663..1588302
• Replicon: chromosome
• Accession: NZ_CP090732
• Organism: Burkholderia cepacia strain 2011Ycf793I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z05_RS30160
• Protein ID: WP_230962748.1
• Coordinates: 1587886..1588302 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2G525
• Locus tag: L0Z05_RS30155
• Protein ID: WP_021159426.1
• Coordinates: 1587663..1587899 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z05_RS30135 (L0Z05_30135)	1582765..1583538	+	774	WP_060341252.1	SDR family oxidoreductase	-
L0Z05_RS30140 (L0Z05_30140)	1583609..1584844	-	1236	WP_060341253.1	acyltransferase	-
L0Z05_RS30145 (L0Z05_30145)	1585141..1585884	-	744	WP_249455293.1	methyltransferase domain-containing protein	-
L0Z05_RS30150 (L0Z05_30150)	1586429..1587266	+	838	Protein_1400	hypothetical protein	-
L0Z05_RS30155 (L0Z05_30155)	1587663..1587899	+	237	WP_021159426.1	hypothetical protein	Antitoxin
L0Z05_RS30160 (L0Z05_30160)	1587886..1588302	+	417	WP_230962748.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z05_RS30165 (L0Z05_30165)	1588502..1589398	+	897	WP_048025080.1	VOC family protein	-
L0Z05_RS30170 (L0Z05_30170)	1589408..1590301	+	894	WP_042975012.1	CoA-transferase	-
L0Z05_RS30175 (L0Z05_30175)	1590312..1591109	+	798	WP_048025082.1	ketoacid CoA transferase	-
L0Z05_RS30180 (L0Z05_30180)	1591118..1592038	+	921	WP_249455294.1	enoyl-CoA hydratase	-
L0Z05_RS30185 (L0Z05_30185)	1592035..1593135	+	1101	WP_059707397.1	nitronate monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15278.57 Da
• Isoelectric Point: 8.5855

>T231746 WP_230962748.1 NZ_CP090732:1587886-1588302 [Burkholderia cepacia]
VYLIDTNVISEIRKGKRTNRGVRAFFRQAAADASPLYLSVVTVAELRRGVDLIRHRGDHPQASALEAWVAMILAGYAPNI
LPVDIEISQTWGHLRVPDPTHELDKLIAATALINDLTVVTRNVADFARTGVRLLNPFD

Nucleotide

• Length: 417 bp

>T231746 NZ_CP126946:4907150-4907461 [Escherichia coli O78:H51]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8681.75 Da
• Isoelectric Point: 5.8214

>AT231746 WP_021159426.1 NZ_CP090732:1587663-1587899 [Burkholderia cepacia]
MANLLVRNVDDNIVQSLRERAAANGRSAEAEHRAILADALGRPKRKTFAQVLMSMPDVGEDADFQRIQDSGEARRVFD

Nucleotide

• Length: 237 bp

>AT231746 NZ_CP126946:4907462-4907752 [Escherichia coli O78:H51]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: U2G525