Detailed information of TA system TA231740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 2253598..2254271
• Replicon: chromosome
• Accession: NZ_CP090730
• Organism: Burkholderia cepacia strain 2011Ycf793I

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: U2G353
• Locus tag: L0Z05_RS10725
• Protein ID: WP_021160096.1
• Coordinates: 2253598..2254014 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: U2FLJ7
• Locus tag: L0Z05_RS10730
• Protein ID: WP_021160097.1
• Coordinates: 2254011..2254271 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z05_RS10705 (L0Z05_10705)	2249311..2249850	-	540	WP_249452222.1	outer membrane protein assembly factor BamE	-
L0Z05_RS10710 (L0Z05_10710)	2250097..2251260	-	1164	WP_027787433.1	M20 aminoacylase family protein	-
L0Z05_RS10715 (L0Z05_10715)	2251602..2252675	+	1074	WP_249453548.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
L0Z05_RS10720 (L0Z05_10720)	2252644..2253549	+	906	WP_104766674.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
L0Z05_RS10725 (L0Z05_10725)	2253598..2254014	-	417	WP_021160096.1	PIN domain-containing protein	Toxin
L0Z05_RS10730 (L0Z05_10730)	2254011..2254271	-	261	WP_021160097.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z05_RS10735 (L0Z05_10735)	2254522..2255538	-	1017	WP_048020596.1	GNAT family N-acetyltransferase	-
L0Z05_RS10740 (L0Z05_10740)	2255538..2256377	-	840	WP_249452223.1	formyltransferase family protein	-
L0Z05_RS10745 (L0Z05_10745)	2256464..2258716	-	2253	WP_249452224.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14566.75 Da
• Isoelectric Point: 7.8895

>T231740 WP_021160096.1 NZ_CP090730:c2254014-2253598 [Burkholderia cepacia]
VTTLYMLDTNICSFIMRERPPVVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVSRLDGVLSWDTA
AVDATAAIRADLAARGMPIGANDASIAGHAIAAGAVLVTNNGREFGRVAGLSLEDWAA

Nucleotide

• Length: 417 bp

>T231740 NZ_CP126946:c3700208-3699801 [Escherichia coli O78:H51]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9546.80 Da
• Isoelectric Point: 4.6151

>AT231740 WP_021160097.1 NZ_CP090730:c2254271-2254011 [Burkholderia cepacia]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSFASEPLADADFLAERPAVIESGRFDLSGGAA
PTESGQ

Nucleotide

• Length: 261 bp

>AT231740 NZ_CP126946:c3700455-3700189 [Escherichia coli O78:H51]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: U2G353

Antitoxin

• AlphaFold DB: U2FLJ7