Detailed information of TA system TA231737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3165735..3166372
• Replicon: chromosome
• Accession: NZ_CP090728
• Organism: Burkholderia multivorans strain 2014Y70439247

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Z30_RS25665
• Protein ID: WP_035489546.1
• Coordinates: 3165971..3166372 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z30_RS25660
• Protein ID: WP_006408845.1
• Coordinates: 3165735..3165971 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z30_RS25640 (L0Z30_25640)	3160896..3161438	+	543	WP_088923753.1	hypothetical protein	-
L0Z30_RS25645 (L0Z30_25645)	3161609..3162640	+	1032	WP_105751888.1	helix-turn-helix domain-containing protein	-
L0Z30_RS25650 (L0Z30_25650)	3162759..3163847	-	1089	WP_105751887.1	ADP-heptose--LPS heptosyltransferase	-
L0Z30_RS25655 (L0Z30_25655)	3163844..3165547	-	1704	WP_105751886.1	thiamine pyrophosphate-binding protein	-
L0Z30_RS25660 (L0Z30_25660)	3165735..3165971	+	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z30_RS25665 (L0Z30_25665)	3165971..3166372	+	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z30_RS25670 (L0Z30_25670)	3166494..3167882	-	1389	WP_105751885.1	L-serine ammonia-lyase	-
L0Z30_RS25675 (L0Z30_25675)	3167909..3169051	-	1143	WP_053296686.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231737 WP_035489546.1 NZ_CP090728:3165971-3166372 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231737 NZ_CP126946:c2741466-2741215 [Escherichia coli O78:H51]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231737 WP_006408845.1 NZ_CP090728:3165735-3165971 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231737 NZ_CP126946:c2741218-2740964 [Escherichia coli O78:H51]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1