Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 2354276..2354909 Replicon chromosome
Accession NZ_CP090728
Organism Burkholderia multivorans strain 2014Y70439247

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag L0Z30_RS21915 Protein ID WP_105767892.1
Coordinates 2354276..2354689 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag L0Z30_RS21920 Protein ID WP_085545763.1
Coordinates 2354676..2354909 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Z30_RS21895 (L0Z30_21895) 2350658..2350987 + 330 WP_105767975.1 hypothetical protein -
L0Z30_RS21900 (L0Z30_21900) 2351000..2352433 + 1434 Protein_2170 conjugal transfer protein TraG N-terminal domain-containing protein -
L0Z30_RS21905 (L0Z30_21905) 2352498..2353757 + 1260 WP_249458451.1 IS256-like element IS1356 family transposase -
L0Z30_RS21910 (L0Z30_21910) 2353879..2354241 - 363 WP_105767921.1 DUF3742 family protein -
L0Z30_RS21915 (L0Z30_21915) 2354276..2354689 - 414 WP_105767892.1 PIN domain-containing protein Toxin
L0Z30_RS21920 (L0Z30_21920) 2354676..2354909 - 234 WP_085545763.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
L0Z30_RS21925 (L0Z30_21925) 2355216..2355911 + 696 WP_105799419.1 phospholipase D family protein -
L0Z30_RS21930 (L0Z30_21930) 2356310..2356972 - 663 WP_105751491.1 uracil-DNA glycosylase -
L0Z30_RS21935 (L0Z30_21935) 2356969..2358108 - 1140 WP_105751490.1 nucleoside triphosphate pyrophosphohydrolase family protein -
L0Z30_RS21940 (L0Z30_21940) 2358112..2358738 - 627 WP_105751489.1 hypothetical protein -
L0Z30_RS21945 (L0Z30_21945) 2358738..2359205 - 468 WP_105751502.1 TIR domain-containing protein -
L0Z30_RS21950 (L0Z30_21950) 2359509..2359760 + 252 Protein_2180 AlpA family phage regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2346695..2359205 12510
- flank IS/Tn - - 2352498..2353757 1259


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-120)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15293.73 Da        Isoelectric Point: 6.0823

>T231736 WP_105767892.1 NZ_CP090728:c2354689-2354276 [Burkholderia multivorans]
MSGADKAKVFVDSNVVLYLLSGDAAKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVDQFLELVRQFCVVAPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCHALYTEDMHHGLIIEESLTLRNPFA

Download         Length: 414 bp

>T231736 NZ_CP126946:c2741218-2740964 [Escherichia coli O78:H51]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8732.97 Da        Isoelectric Point: 7.0164

>AT231736 WP_085545763.1 NZ_CP090728:c2354909-2354676 [Burkholderia multivorans]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVIDAPRTFAVRKKPGADELLERLRHFRGKLPADFKFSRDEANERG

Download         Length: 234 bp

>AT231736 NZ_CP126946:c2741466-2741215 [Escherichia coli O78:H51]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References