Detailed information of TA system TA231735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 1022997..1023697
• Replicon: chromosome
• Accession: NZ_CP090728
• Organism: Burkholderia multivorans strain 2014Y70439247

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8E2RY82
• Locus tag: L0Z30_RS15735
• Protein ID: WP_070553238.1
• Coordinates: 1023320..1023697 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: L0Z30_RS15730
• Protein ID: WP_006405635.1
• Coordinates: 1022997..1023320 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z30_RS15710 (L0Z30_15710)	1018346..1019806	+	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-
L0Z30_RS15715 (L0Z30_15715)	1019965..1021863	+	1899	WP_059875776.1	molecular chaperone HtpG	-
L0Z30_RS15720 (L0Z30_15720)	1021872..1022486	+	615	WP_006398690.1	chorismate lyase	-
L0Z30_RS15725 (L0Z30_15725)	1022483..1022974	+	492	WP_006398691.1	DNA-deoxyinosine glycosylase	-
L0Z30_RS15730 (L0Z30_15730)	1022997..1023320	-	324	WP_006405635.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z30_RS15735 (L0Z30_15735)	1023320..1023697	-	378	WP_070553238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z30_RS15740 (L0Z30_15740)	1024227..1024481	-	255	WP_059940739.1	hypothetical protein	-
L0Z30_RS15745 (L0Z30_15745)	1024709..1025626	+	918	WP_053296988.1	spermidine synthase	-
L0Z30_RS15750 (L0Z30_15750)	1025823..1026056	+	234	WP_006398697.1	DUF3311 domain-containing protein	-
L0Z30_RS15755 (L0Z30_15755)	1026053..1027603	+	1551	WP_006398698.1	sodium:solute symporter family protein	-
L0Z30_RS15760 (L0Z30_15760)	1027717..1028166	-	450	WP_105751509.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13507.49 Da
• Isoelectric Point: 9.3081

>T231735 WP_070553238.1 NZ_CP090728:c1023697-1023320 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGEK

Nucleotide

• Length: 378 bp

>T231735 NZ_CP126946:2726150-2726524 [Escherichia coli O78:H51]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCATCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT231735 WP_006405635.1 NZ_CP090728:c1023320-1022997 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT231735 NZ_CP126946:2725693-2726061 [Escherichia coli O78:H51]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCATACCATCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: B9BQB8