Detailed information of TA system TA231734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1819474..1820744
• Replicon: chromosome
• Accession: NZ_CP090727
• Organism: Burkholderia multivorans strain 2014Y70439247

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z30_RS08225
• Protein ID: WP_105751379.1
• Coordinates: 1820166..1820744 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BS12
• Locus tag: L0Z30_RS08220
• Protein ID: WP_006406126.1
• Coordinates: 1819474..1820031 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z30_RS08205 (L0Z30_08205)	1815533..1816792	-	1260	WP_006401211.1	IS256-like element IS1356 family transposase	-
L0Z30_RS08210 (L0Z30_08210)	1816927..1817547	+	621	WP_006410617.1	isochorismatase family cysteine hydrolase	-
L0Z30_RS08215 (L0Z30_08215)	1817558..1818961	-	1404	WP_088929364.1	hypothetical protein	-
L0Z30_RS08220 (L0Z30_08220)	1819474..1820031	+	558	WP_006406126.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z30_RS08225 (L0Z30_08225)	1820166..1820744	+	579	WP_105751379.1	HD domain-containing protein	Toxin
L0Z30_RS08230 (L0Z30_08230)	1820851..1822389	-	1539	WP_105751380.1	MFS transporter	-
L0Z30_RS08235 (L0Z30_08235)	1822568..1823488	+	921	WP_065496151.1	LysR family transcriptional regulator	-
L0Z30_RS08240 (L0Z30_08240)	1823535..1824674	-	1140	WP_006410588.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z30_RS08245 (L0Z30_08245)	1824671..1825462	-	792	WP_035947285.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1815533..1816792	1259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-163)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21416.15 Da
• Isoelectric Point: 4.9434

>T231734 WP_105751379.1 NZ_CP090727:1820166-1820744 [Burkholderia multivorans]
MNPDTIRVRLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
ATEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDAAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAQT

Nucleotide

• Length: 579 bp

>T231734 NZ_CP126946:c2488857-2488754 [Escherichia coli O78:H51]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20378.30 Da
• Isoelectric Point: 7.8341

>AT231734 WP_006406126.1 NZ_CP090727:1819474-1820031 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAAHLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMETALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231734 NZ_CP126946:2488716-2488987 [Escherichia coli O78:H51]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: B9BS12