Detailed information of TA system TA231728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1799000..1800270
• Replicon: chromosome
• Accession: NZ_CP090725
• Organism: Burkholderia multivorans strain 2014Y70518672I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z29_RS23795
• Protein ID: WP_105751379.1
• Coordinates: 1799692..1800270 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BS12
• Locus tag: L0Z29_RS23790
• Protein ID: WP_006406126.1
• Coordinates: 1799000..1799557 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z29_RS23775 (L0Z29_23775)	1795059..1796318	-	1260	WP_006401211.1	IS256-like element IS1356 family transposase	-
L0Z29_RS23780 (L0Z29_23780)	1796453..1797073	+	621	WP_006410617.1	isochorismatase family cysteine hydrolase	-
L0Z29_RS23785 (L0Z29_23785)	1797084..1798487	-	1404	WP_088929364.1	hypothetical protein	-
L0Z29_RS23790 (L0Z29_23790)	1799000..1799557	+	558	WP_006406126.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z29_RS23795 (L0Z29_23795)	1799692..1800270	+	579	WP_105751379.1	HD domain-containing protein	Toxin
L0Z29_RS23800 (L0Z29_23800)	1800377..1801915	-	1539	WP_105751380.1	MFS transporter	-
L0Z29_RS23805 (L0Z29_23805)	1802094..1803014	+	921	WP_065496151.1	LysR family transcriptional regulator	-
L0Z29_RS23810 (L0Z29_23810)	1803061..1804200	-	1140	WP_006410588.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z29_RS23815 (L0Z29_23815)	1804197..1804988	-	792	WP_035947285.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1795059..1796318	1259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-163)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21416.15 Da
• Isoelectric Point: 4.9434

>T231728 WP_105751379.1 NZ_CP090725:1799692-1800270 [Burkholderia multivorans]
MNPDTIRVRLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
ATEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDAAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAQT

Nucleotide

• Length: 579 bp

>T231728 NZ_CP126946:c1005894-1005676 [Escherichia coli O78:H51]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20378.30 Da
• Isoelectric Point: 7.8341

>AT231728 WP_006406126.1 NZ_CP090725:1799000-1799557 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAAHLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMETALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231728 NZ_CP126946:c1006294-1005920 [Escherichia coli O78:H51]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: B9BS12