Detailed information of TA system TA231727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1636752..1637407
• Replicon: chromosome
• Accession: NZ_CP090725
• Organism: Burkholderia multivorans strain 2014Y70518672I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z29_RS23120
• Protein ID: WP_006403072.1
• Coordinates: 1636752..1637171 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: L0Z29_RS23125
• Protein ID: WP_006403071.1
• Coordinates: 1637168..1637407 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z29_RS23095 (L0Z29_23095)	1631921..1632715	+	795	WP_006403076.1	AraC family transcriptional regulator	-
L0Z29_RS23100 (L0Z29_23100)	1633142..1634092	+	951	WP_105752205.1	DMT family transporter	-
L0Z29_RS23105 (L0Z29_23105)	1634287..1634862	+	576	WP_006403074.1	hypothetical protein	-
L0Z29_RS23110 (L0Z29_23110)	1634956..1635090	-	135	WP_006396654.1	entericidin A/B family lipoprotein	-
L0Z29_RS23115 (L0Z29_23115)	1635323..1636618	+	1296	WP_035487510.1	aspartate carbamoyltransferase	-
L0Z29_RS23120 (L0Z29_23120)	1636752..1637171	-	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z29_RS23125 (L0Z29_23125)	1637168..1637407	-	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
L0Z29_RS23130 (L0Z29_23130)	1637711..1639957	+	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z29_RS23135 (L0Z29_23135)	1639961..1640716	+	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z29_RS23140 (L0Z29_23140)	1640713..1641396	+	684	WP_006396647.1	Fe2+-dependent dioxygenase	-
L0Z29_RS23145 (L0Z29_23145)	1641410..1641613	-	204	WP_006403067.1	hypothetical protein	-
L0Z29_RS23150 (L0Z29_23150)	1642202..1642345	+	144	WP_006409932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231727 WP_006403072.1 NZ_CP090725:c1637171-1636752 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231727 NZ_CP126946:c971922-971380 [Escherichia coli O78:H51]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT231727 WP_006403071.1 NZ_CP090725:c1637407-1637168 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT231727 NZ_CP126946:c972217-971906 [Escherichia coli O78:H51]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6