Detailed information of TA system TA231722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2614341..2614974
• Replicon: chromosome
• Accession: NZ_CP090724
• Organism: Burkholderia multivorans strain 2014Y70518672I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z29_RS12255
• Protein ID: WP_105767892.1
• Coordinates: 2614341..2614754 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z29_RS12260
• Protein ID: WP_085545763.1
• Coordinates: 2614741..2614974 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z29_RS12235 (L0Z29_12235)	2610723..2611052	+	330	WP_105767975.1	hypothetical protein	-
L0Z29_RS12240 (L0Z29_12240)	2611065..2612498	+	1434	Protein_2397	conjugal transfer protein TraG N-terminal domain-containing protein	-
L0Z29_RS12245 (L0Z29_12245)	2612563..2613822	+	1260	WP_249458451.1	IS256-like element IS1356 family transposase	-
L0Z29_RS12250 (L0Z29_12250)	2613944..2614306	-	363	WP_105767921.1	DUF3742 family protein	-
L0Z29_RS12255 (L0Z29_12255)	2614341..2614754	-	414	WP_105767892.1	PIN domain-containing protein	Toxin
L0Z29_RS12260 (L0Z29_12260)	2614741..2614974	-	234	WP_085545763.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Z29_RS12265 (L0Z29_12265)	2615281..2615976	+	696	WP_105799419.1	phospholipase D family protein	-
L0Z29_RS12270 (L0Z29_12270)	2616375..2617037	-	663	WP_105751491.1	uracil-DNA glycosylase	-
L0Z29_RS12275 (L0Z29_12275)	2617034..2618173	-	1140	WP_105751490.1	nucleoside triphosphate pyrophosphohydrolase family protein	-
L0Z29_RS12280 (L0Z29_12280)	2618177..2618803	-	627	WP_105751489.1	hypothetical protein	-
L0Z29_RS12285 (L0Z29_12285)	2618803..2619270	-	468	WP_105751502.1	TIR domain-containing protein	-
L0Z29_RS12290 (L0Z29_12290)	2619574..2619825	+	252	Protein_2407	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2612563..2613822	1259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15293.73 Da
• Isoelectric Point: 6.0823

>T231722 WP_105767892.1 NZ_CP090724:c2614754-2614341 [Burkholderia multivorans]
MSGADKAKVFVDSNVVLYLLSGDAAKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVDQFLELVRQFCVVAPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCHALYTEDMHHGLIIEESLTLRNPFA

Nucleotide

• Length: 414 bp

>T231722 NZ_CP126945:134109-134396 [Escherichia coli O1:K1:H7]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8732.97 Da
• Isoelectric Point: 7.0164

>AT231722 WP_085545763.1 NZ_CP090724:c2614974-2614741 [Burkholderia multivorans]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVIDAPRTFAVRKKPGADELLERLRHFRGKLPADFKFSRDEANERG

Nucleotide

• Length: 234 bp

>AT231722 NZ_CP126945:133861-134112 [Escherichia coli O1:K1:H7]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA