Detailed information of TA system TA231718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1509318..1509895
• Replicon: chromosome
• Accession: NZ_CP090722
• Organism: Burkholderia multivorans strain 2014Y70522077

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z19_RS22435
• Protein ID: WP_249461355.1
• Coordinates: 1509530..1509895 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z19_RS22430
• Protein ID: WP_035956044.1
• Coordinates: 1509318..1509536 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z19_RS22410 (L0Z19_22410)	1504957..1506228	-	1272	WP_249461346.1	Zn-dependent hydrolase	-
L0Z19_RS22415 (L0Z19_22415)	1506221..1506760	-	540	WP_249461349.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z19_RS22420 (L0Z19_22420)	1506978..1508144	-	1167	WP_249461352.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z19_RS22425 (L0Z19_22425)	1508320..1509216	+	897	WP_105794177.1	LysR substrate-binding domain-containing protein	-
L0Z19_RS22430 (L0Z19_22430)	1509318..1509536	+	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z19_RS22435 (L0Z19_22435)	1509530..1509895	+	366	WP_249461355.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z19_RS22440 (L0Z19_22440)	1509991..1510179	+	189	WP_200053152.1	hypothetical protein	-
L0Z19_RS22445 (L0Z19_22445)	1510218..1510376	-	159	WP_200053153.1	hypothetical protein	-
L0Z19_RS22450 (L0Z19_22450)	1510626..1511189	+	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z19_RS22455 (L0Z19_22455)	1511308..1512906	+	1599	WP_249461358.1	alkyl hydroperoxide reductase subunit F	-
L0Z19_RS22460 (L0Z19_22460)	1512968..1513708	-	741	WP_249461361.1	hypothetical protein	-
L0Z19_RS22465 (L0Z19_22465)	1513832..1514731	-	900	WP_249461365.1	lipid A hydroxylase LpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13625.66 Da
• Isoelectric Point: 5.8683

>T231718 WP_249461355.1 NZ_CP090722:1509530-1509895 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDITVADRAVTIRKER
RIRLPDAIVWASAQVNGRLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231718 NZ_CP126945:c72815-72399 [Escherichia coli O1:K1:H7]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231718 WP_035956044.1 NZ_CP090722:1509318-1509536 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231718 NZ_CP126945:c73042-72812 [Escherichia coli O1:K1:H7]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45