Detailed information of TA system TA231716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 769070..769725
• Replicon: chromosome
• Accession: NZ_CP090722
• Organism: Burkholderia multivorans strain 2014Y70522077

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z19_RS19190
• Protein ID: WP_006409935.1
• Coordinates: 769306..769725 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z19_RS19185
• Protein ID: WP_249462451.1
• Coordinates: 769070..769309 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z19_RS19160 (L0Z19_19160)	764131..764274	-	144	WP_006409932.1	hypothetical protein	-
L0Z19_RS19165 (L0Z19_19165)	764863..765066	+	204	WP_006403067.1	hypothetical protein	-
L0Z19_RS19170 (L0Z19_19170)	765093..765764	-	672	Protein_630	Fe2+-dependent dioxygenase	-
L0Z19_RS19175 (L0Z19_19175)	765761..766516	-	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z19_RS19180 (L0Z19_19180)	766520..768766	-	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z19_RS19185 (L0Z19_19185)	769070..769309	+	240	WP_249462451.1	Arc family DNA-binding protein	Antitoxin
L0Z19_RS19190 (L0Z19_19190)	769306..769725	+	420	WP_006409935.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z19_RS19195 (L0Z19_19195)	769859..771154	-	1296	WP_249462452.1	aspartate carbamoyltransferase	-
L0Z19_RS19200 (L0Z19_19200)	771387..771521	+	135	WP_006409923.1	entericidin A/B family lipoprotein	-
L0Z19_RS19205 (L0Z19_19205)	771615..772190	-	576	WP_053298362.1	hypothetical protein	-
L0Z19_RS19210 (L0Z19_19210)	772386..773336	-	951	WP_217057526.1	DMT family transporter	-
L0Z19_RS19215 (L0Z19_19215)	773582..774376	-	795	WP_105777245.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15146.38 Da
• Isoelectric Point: 5.2157

>T231716 WP_006409935.1 NZ_CP090722:769306-769725 [Burkholderia multivorans]
MILVDTNVIFEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231716 NZ_CP126944:c4933220-4932909 [Escherichia coli O1:K1:H7]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8788.06 Da
• Isoelectric Point: 5.7066

>AT231716 WP_249462451.1 NZ_CP090722:769070-769309 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSIEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTRTDPMSFE

Nucleotide

• Length: 240 bp

>AT231716 NZ_CP126944:c4932908-4932618 [Escherichia coli O1:K1:H7]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG