Detailed information of TA system TA231709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1370867..1371906
• Replicon: chromosome
• Accession: NZ_CP090717
• Organism: Burkholderia multivorans strain 2014Y70532416II

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A1W0YRD2
• Locus tag: L0Y81_RS05865
• Protein ID: WP_060096181.1
• Coordinates: 1370867..1371442 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: L0Y81_RS05870
• Protein ID: WP_054316743.1
• Coordinates: 1371439..1371906 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y81_RS05835 (L0Y81_05835)	1366257..1367339	+	1083	WP_006415045.1	porin	-
L0Y81_RS05840 (L0Y81_05840)	1367475..1368125	-	651	WP_244130927.1	hypothetical protein	-
L0Y81_RS05845 (L0Y81_05845)	1368253..1368363	+	111	Protein_1161	porin	-
L0Y81_RS05850 (L0Y81_05850)	1368422..1369387	-	966	WP_105836659.1	zinc-binding alcohol dehydrogenase family protein	-
L0Y81_RS05855 (L0Y81_05855)	1369466..1369717	-	252	WP_039218251.1	hypothetical protein	-
L0Y81_RS05860 (L0Y81_05860)	1369813..1370622	+	810	WP_105836660.1	helix-turn-helix transcriptional regulator	-
L0Y81_RS05865 (L0Y81_05865)	1370867..1371442	-	576	WP_060096181.1	PIN domain-containing protein	Toxin
L0Y81_RS05870 (L0Y81_05870)	1371439..1371906	-	468	WP_054316743.1	helix-turn-helix domain-containing protein	Antitoxin
L0Y81_RS05875 (L0Y81_05875)	1372022..1372828	-	807	WP_105836661.1	4-hydroxy-2-oxoheptanedioate aldolase	-
L0Y81_RS05880 (L0Y81_05880)	1372836..1373639	-	804	WP_105842135.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
L0Y81_RS05885 (L0Y81_05885)	1373654..1374046	-	393	WP_006403681.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
L0Y81_RS05890 (L0Y81_05890)	1374082..1374930	-	849	WP_012217098.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
L0Y81_RS05895 (L0Y81_05895)	1374973..1376436	-	1464	WP_105836663.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21554.67 Da
• Isoelectric Point: 5.0551

>T231709 WP_060096181.1 NZ_CP090717:c1371442-1370867 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLAAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDNLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T231709 NZ_CP126944:3693903-3694121 [Escherichia coli O1:K1:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17581.02 Da
• Isoelectric Point: 6.1443

>AT231709 WP_054316743.1 NZ_CP090717:c1371906-1371439 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEGQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELGKQAQALRMGYE

Nucleotide

• Length: 468 bp

>AT231709 NZ_CP126944:3693503-3693877 [Escherichia coli O1:K1:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1W0YRD2