Detailed information of TA system TA231701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1395095..1396134
• Replicon: chromosome
• Accession: NZ_CP090715
• Organism: Burkholderia multivorans strain 2015Y70629342III

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: L0Z12_RS23150
• Protein ID: WP_105751292.1
• Coordinates: 1395095..1395670 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A8E2S2M5
• Locus tag: L0Z12_RS23155
• Protein ID: WP_053298663.1
• Coordinates: 1395667..1396134 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z12_RS23115 (L0Z12_23115)	1390171..1391253	+	1083	WP_006403670.1	porin	-
L0Z12_RS23120 (L0Z12_23120)	1391389..1391889	-	501	WP_249482023.1	hypothetical protein	-
L0Z12_RS23125 (L0Z12_23125)	1391886..1392125	-	240	WP_244131642.1	DUF6402 family protein	-
L0Z12_RS23130 (L0Z12_23130)	1392477..1392590	+	114	Protein_1204	porin	-
L0Z12_RS23135 (L0Z12_23135)	1392649..1393614	-	966	WP_105751295.1	zinc-binding alcohol dehydrogenase family protein	-
L0Z12_RS23140 (L0Z12_23140)	1393694..1393945	-	252	WP_105751294.1	hypothetical protein	-
L0Z12_RS23145 (L0Z12_23145)	1394041..1394850	+	810	WP_105751293.1	helix-turn-helix transcriptional regulator	-
L0Z12_RS23150 (L0Z12_23150)	1395095..1395670	-	576	WP_105751292.1	PIN domain-containing protein	Toxin
L0Z12_RS23155 (L0Z12_23155)	1395667..1396134	-	468	WP_053298663.1	helix-turn-helix domain-containing protein	Antitoxin
L0Z12_RS23160 (L0Z12_23160)	1396250..1397056	-	807	WP_065495028.1	4-hydroxy-2-oxoheptanedioate aldolase	-
L0Z12_RS23165 (L0Z12_23165)	1397064..1397867	-	804	WP_006402377.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
L0Z12_RS23170 (L0Z12_23170)	1397882..1398274	-	393	WP_217056988.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
L0Z12_RS23175 (L0Z12_23175)	1398310..1399158	-	849	WP_088929843.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
L0Z12_RS23180 (L0Z12_23180)	1399201..1400664	-	1464	WP_249482024.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pmlI/bspI1 / pmlR/bspR1	933057..1403904	470847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21585.68 Da
• Isoelectric Point: 4.8924

>T231701 WP_105751292.1 NZ_CP090715:c1395670-1395095 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLTAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDDLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T231701 NZ_CP126944:1192419-1192754 [Escherichia coli O1:K1:H7]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17669.09 Da
• Isoelectric Point: 5.8709

>AT231701 WP_053298663.1 NZ_CP090715:c1396134-1395667 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEAQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELSKQAQDLRMGYE

Nucleotide

• Length: 468 bp

>AT231701 NZ_CP126944:1192171-1192419 [Escherichia coli O1:K1:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA