Detailed information of TA system TA231698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 823193..823848
• Replicon: chromosome
• Accession: NZ_CP090715
• Organism: Burkholderia multivorans strain 2015Y70629342III

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z12_RS20575
• Protein ID: WP_006403072.1
• Coordinates: 823429..823848 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: L0Z12_RS20570
• Protein ID: WP_006403071.1
• Coordinates: 823193..823432 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z12_RS20545 (L0Z12_20545)	818255..818398	-	144	WP_006409932.1	hypothetical protein	-
L0Z12_RS20550 (L0Z12_20550)	818987..819190	+	204	WP_006403067.1	hypothetical protein	-
L0Z12_RS20555 (L0Z12_20555)	819204..819887	-	684	WP_006396647.1	Fe2+-dependent dioxygenase	-
L0Z12_RS20560 (L0Z12_20560)	819884..820639	-	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z12_RS20565 (L0Z12_20565)	820643..822889	-	2247	WP_249482126.1	TonB-dependent siderophore receptor	-
L0Z12_RS20570 (L0Z12_20570)	823193..823432	+	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
L0Z12_RS20575 (L0Z12_20575)	823429..823848	+	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z12_RS20580 (L0Z12_20580)	823982..825277	-	1296	WP_035487510.1	aspartate carbamoyltransferase	-
L0Z12_RS20585 (L0Z12_20585)	825510..825644	+	135	WP_006396654.1	entericidin A/B family lipoprotein	-
L0Z12_RS20590 (L0Z12_20590)	825738..826313	-	576	WP_006403074.1	hypothetical protein	-
L0Z12_RS20595 (L0Z12_20595)	826508..827458	-	951	WP_105752205.1	DMT family transporter	-
L0Z12_RS20600 (L0Z12_20600)	827885..828679	-	795	WP_006403076.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231698 WP_006403072.1 NZ_CP090715:823429-823848 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231698 NZ_CP126944:c842298-841921 [Escherichia coli O1:K1:H7]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT231698 WP_006403071.1 NZ_CP090715:823193-823432 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT231698 NZ_CP126944:c842722-842345 [Escherichia coli O1:K1:H7]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6