Detailed information of TA system TA231694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2162331..2162964
• Replicon: chromosome
• Accession: NZ_CP090714
• Organism: Burkholderia multivorans strain 2015Y70629342III

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z12_RS10365
• Protein ID: WP_105767892.1
• Coordinates: 2162331..2162744 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z12_RS10370
• Protein ID: WP_085545763.1
• Coordinates: 2162731..2162964 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z12_RS10340 (L0Z12_10340)	2158347..2158670	+	324	WP_244131747.1	TIGR03757 family integrating conjugative element protein	-
L0Z12_RS10345 (L0Z12_10345)	2158667..2159266	+	600	Protein_2015	TIGR03756 family integrating conjugative element protein	-
L0Z12_RS10350 (L0Z12_10350)	2159262..2160488	+	1227	Protein_2016	conjugal transfer protein TraG N-terminal domain-containing protein	-
L0Z12_RS10355 (L0Z12_10355)	2160553..2161812	+	1260	WP_249458451.1	IS256-like element IS1356 family transposase	-
L0Z12_RS10360 (L0Z12_10360)	2161934..2162296	-	363	WP_105767921.1	DUF3742 family protein	-
L0Z12_RS10365 (L0Z12_10365)	2162331..2162744	-	414	WP_105767892.1	PIN domain-containing protein	Toxin
L0Z12_RS10370 (L0Z12_10370)	2162731..2162964	-	234	WP_085545763.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Z12_RS30680	2163671..2163796	+	126	WP_276523945.1	hypothetical protein	-
L0Z12_RS10375 (L0Z12_10375)	2163869..2165067	+	1199	WP_163013128.1	IS3-like element ISButh1 family transposase	-
L0Z12_RS10380 (L0Z12_10380)	2165132..2166265	+	1134	Protein_2023	IS256-like element IS1356 family transposase	-
L0Z12_RS10385 (L0Z12_10385)	2166839..2167102	-	264	WP_146122407.1	hypothetical protein	-
L0Z12_RS10390 (L0Z12_10390)	2167065..2167640	-	576	WP_249481702.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2154668..2169969	15301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15293.73 Da
• Isoelectric Point: 6.0823

>T231694 WP_105767892.1 NZ_CP090714:c2162744-2162331 [Burkholderia multivorans]
MSGADKAKVFVDSNVVLYLLSGDAAKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVDQFLELVRQFCVVAPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCHALYTEDMHHGLIIEESLTLRNPFA

Nucleotide

• Length: 414 bp

>T231694 NZ_CP126943:91356-91772 [Escherichia coli O1:K1:H7]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8732.97 Da
• Isoelectric Point: 7.0164

>AT231694 WP_085545763.1 NZ_CP090714:c2162964-2162731 [Burkholderia multivorans]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVIDAPRTFAVRKKPGADELLERLRHFRGKLPADFKFSRDEANERG

Nucleotide

• Length: 234 bp

>AT231694 NZ_CP126943:91129-91359 [Escherichia coli O1:K1:H7]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA